@article{Zapata_Durán_Stashenko_Correa-Royero_Betancur-Galvis_2009, title={Actividad citotóxica de aceites esenciales de Lippia origanoides H.B.K. y componentes mayoritarios}, volume={41}, url={https://revistas.uis.edu.co/index.php/revistasaluduis/article/view/680}, abstractNote={<p style="text-align: center;" align="center"><strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;">RESUMEN </span></strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;"></span></p><p style="text-align: justify;"><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;">INTRODUCCIÓN: <em><span style="font-family: ";Verdana";,";sans-serif";;">Lippia origanoides </span></em>H.B.K. (Verbenacea), es una planta aromática conocida comúnmente como "orégano". Los aceites esenciales de 8 muestras de <em><span style="font-family: ";Verdana";,";sans-serif";;">L</span></em>. <em><span style="font-family: ";Verdana";,";sans-serif";;">origanoides </span></em>y algunos de sus componentes mayoritarios fueron evaluados <em><span style="font-family: ";Verdana";,";sans-serif";;">in vitro </span></em>sobre la línea tumoral HeLa y la línea no tumoral Vero para identifcar su potencial citotóxico. </span></p><p style="text-align: justify;"><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;">MATERIALES Y MÉTODOS: la concentración inhibitoria cincuenta (IC<sub>50</sub>) se determinó mediante la técnica fotocolorimétrica del MTT (3-(4,5-Dimetiltiazol-2-il)-2,5-bromuro difeniltetrazolio) y los valores de IC<sub>50</sub> se obtuvieron por análisis estadístico mediante regresión lineal simple. El índice de selectividad (IS), defnido como la IC<sub>50</sub> en células Vero sobre IC<sub>50</sub> en células HeLa, fue calculado con el fn de encontrar aceites o componentes con potencial citotóxico selectivo hacia líneas celulares tumorales. </span></p><p style="text-align: justify;"><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;">RESULTADOS Y CONCLUSIONES:<strong><span style="font-family: ";Verdana";,";sans-serif";;"> </span></strong>se determinó por cromatografía de gases y espectrometría de masas GC/MS la composición química de los aceites más citotóxicos. El aceite de <em><span style="font-family: ";Verdana";,";sans-serif";;">L. origanoides </span></em>que presentó la mayor actividad citotóxica sobre células HeLa con un valor de IC<sub>50</sub> de 9,1 ± 1 µg/mL e índice de selectividad de 7,1, fue identifcado como quimiotipo trans-β-cariofleno/<em><span style="font-family: ";Verdana";,";sans-serif";;">ρ</span></em>-cimeno. Los componentes mayoritarios del aceite quimiotipo trans-β-cariofleno/<em><span style="font-family: ";Verdana";,";sans-serif";;">ρ</span></em>-cimeno fueron: trans-β-cariofleno (11,3%), <em><span style="font-family: ";Verdana";,";sans-serif";;">ρ</span></em>-cimeno (11,2%), α-felandreno (9,9%), limoneno (7,2%), 1,8-cineol (6,5%) y α-humuleno (6,0%). Los componentes mayoritarios evaluados no mostraron actividad citotóxica relevante sobre células HeLa, sólo el limoneno y β-mirceno presentaron valores de IS, respectivamente, de 6,97 y 3,01. Sin embargo, los valores de IC<sub>50</sub> fueron más altos que el del aceite activo. Estos resultados sugieren que la actividad citotóxica de los aceites no se debe sólo a sus componentes mayoritarios, sino a un sinergismo entre sus componentes.<strong><span style="font-family: ";Verdana";,";sans-serif";;"> </span></strong></span></p><p style="text-align: justify;"><strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;">Palabras clave: </span></strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;">Citotoxicidad, Vero, HeLa, <em><span style="font-family: ";Verdana";,";sans-serif";;">Lippia origanoides</span></em>, aceites esenciales, quimiotipos </span></p><p style="text-align: center;" align="center"><strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt;">ABSTRACT</span></strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt; mso-ansi-language: EN-US;" lang="EN-US"> </span></p><p style="text-align: justify;"><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt; mso-ansi-language: EN-US;" lang="EN-US">INTRODUCTION: <em><span style="font-family: ";Verdana";,";sans-serif";;">Lippia origanoides </span></em>H.B.K. (Verbenaceae) is an aromatic plant commonly called as "orégano". Eight essential oils of <em><span style="font-family: ";Verdana";,";sans-serif";;">L. origanoides </span></em>and some of their main components were evaluated <em><span style="font-family: ";Verdana";,";sans-serif";;">in vitro </span></em>on tumor cell line HeLa and non-tumor cell line Vero to identify tumoural cytotoxic potential. </span></p><p style="text-align: justify;"><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt; mso-ansi-language: EN-US;" lang="EN-US">MATERIALS AND METHODS: Inhibition 50% of cell population (IC<sub>50</sub>) was determined using the photo-colorimeter technique MTT (3 - (4.5-dimethylthiazol-2-yl) -2,5-difeniltetrazolium bromide). IC<sub>50</sub> valúes were obtained by linear regression analysis. The selectivity index (SI), defned as Vero IC<sub>50</sub> on HeLa IC<sub>50</sub>, it was calculated in order to fnd oil or major components with selective tumor cytotoxic potential. </span></p><p style="text-align: justify;"><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt; mso-ansi-language: EN-US;" lang="EN-US">RESULTS AND CONCLUSIONS:<strong><span style="font-family: ";Verdana";,";sans-serif";;"> </span></strong>the chemical composition of the oil most cytotoxic was determined by gas chromatography and mass spectrometry GC/MS. The <em><span style="font-family: ";Verdana";,";sans-serif";;">L. origanoides </span></em>oil identifed as chemotype <em><span style="font-family: ";Verdana";,";sans-serif";;">trans-f>-</span></em>caryophyllene/<em><span style="font-family: ";Verdana";,";sans-serif";;">p-</span></em>cymene showed the highest cytotoxic activity on HeLa cells with IC<sub>50</sub> value of 9.1 ± 1 µg/mL and selectivity index of 7.1. The main components were: trans-P-caryophyllene (11.3/>cymene (11.2%), a-phellandrene (9.9%), limonene (7.2%), 1.8-cineol (6.5%) and a-humulene (6.0%). The most of the major components did not show cytotoxic activity on HeLa cells, only limonene and (3-myrcene showed IS valúes of 6.97 and 3.01, respectively. However, the IC<sub>50</sub> values were higher than active oil. These results suggest that cytotoxic activities of the oils are not only due to their main components, but to a synergism among its components.<strong><span style="font-family: ";Verdana";,";sans-serif";;"> </span></strong></span></p><p style="text-align: justify;"><strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt; mso-ansi-language: EN-US;" lang="EN-US">Keywords: </span></strong><span style="font-family: ";Verdana";,";sans-serif";; color: black; font-size: 7.5pt; mso-ansi-language: EN-US;" lang="EN-US">Cytotoxicity, Vero, HeLa, <em><span style="font-family: ";Verdana";,";sans-serif";;">Lippia origanoides, </span></em>essential oil, chemotype</span></p>}, number={3}, journal={Salud UIS}, author={Zapata, Bibiana and Durán, Camilo and Stashenko, Elena and Correa-Royero, Julieth and Betancur-Galvis, Liliana}, year={2009}, month={dic.} }