Efectos de algunos parámetros de reacción en la producción de ésteres etílicos empleando una combi-lipasa de TLL y CALB en Lewatit VP OC 1600

  • Esteban Camilo Toro Alzate Estudiante de Maestría
  • César A. Godoy Universidad del Valle

Resumen

La optimización de mezclas de lipasas inmovilizadas (combi-lipasas) ha sido aplicada como estrategia para incrementar la velocidad y el rendimiento en la producción de ésteres alquílicos de ácidos grasos o en aceites vegetales hidrolizados con respecto a la catálisis de las lipasas individuales; según estudios previos, se identificó la Combi-lipasa (CL) de TLL (75%) y CALB (25%) en LW como la más activa de acuerdo a la cantidad de proteína inmovilizada y su eficiencia en la producción de ésteres etílicos (EE) en condiciones suaves de reacción, sin embargo, su estabilidad en diferentes condiciones no ha sido estudiada. Se determinó que esta CL produce EE de modo estable a diferentes temperaturas y ante la presencia de agua y ácidos grasos libres, sin embargo, la adición de glicerol generó disminuciones de hasta el 70% en la síntesis de ésteres etílicos. Aplicando el modelo de Arrhenius, se determinó tiene una Ea de 21.4 kJ/mol, significando una disminución del 5% frente a su componente principal (TLL-LW), obteniendo mejores rendimientos en tiempos más cortos; adicionalmente, el uso de un aceites de diferente naturaleza como el de soya generó un aumento del 20% en la producción de EE. Por último, se realizó un estudio de docking y la simulación cinética del sistema usando un modelo desarrollado previamente, pero no se logró una clara correlación con los resultados experimentales. Al margen de esto, por su robustez la CL estudiada presenta un alto potencial de aplicación  en síntesis de biodiesel.

Palabras clave: Biocatalizadores, Combi-lipasas, Oleína de palma, Docking, Ésteres Etílicos, Enzimas

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Publicado
2021-01-01
Sección
Artículos