Inmobilización de lipasa en biochar obtenida de Manihot Esculenta Crantz

  • Lívia Maria de Oliveira Ribeiro Laboratório de Processos (LAPRO), Centro de Tecnologia, Universidade Federal de Alagoas
  • Lucas Meili Laboratório de Processos (LAPRO), Centro de Tecnologia, Universidade Federal de Alagoas
  • Geórgia N.S.B. Góis Laboratório de Processos (LAPRO), Centro de Tecnologia, Universidade Federal de Alagoas
  • Renata Maria Rosas Garcia Almeida Centro de Tecnologia, Universidade Federal de Alagoas
  • Jose Leandro da Silva Duarte Laboratório de Processos (LAPRO), Centro de Tecnologia, Universidade Federal de Alagoas

Resumen

Las enzimas inmovilizadas son catalizadores de gran interés industrial, ya que unen las ventajas de la catálisis heterogénea con la alta selectividad y las condiciones de operación suaves de las enzimas. El biochar es un material carbonoso poroso, que tiene la característica de que es un fuerte candidato para incorporar enzimas en su estructura. Otro hecho relevante es la posibilidad de cambios y adaptaciones por condición de síntesis en la estructura de Biochar debido a las necesidades de la aplicación. En este contexto, el objetivo de este trabajo fue el estudio de la inmovilización de lipasa en un biochar producido por pirólisis (600 °C) de un residuo agrícola llamado tocón de yuca. Se evaluaron diferentes concentraciones enzimáticas (0,1, 0,25 y 0,5 g / L) para determinar la afinidad entre la enzima y el biochar. Los resultados obtenidos mostraron que la enzima estudiada se podía inmovilizar fácilmente en el biochar, obteniendo como rendimiento de inmovilización 61,2 % cuando se usa la mayor concentración de enzima.

Palabras clave: Enzima, Adsorción, Material Poroso

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Publicado
2019-11-12
Sección
Artículos