Vol. 34 Núm. 2 (2021): Revista ION
Artículos

Biosíntesis de nanopartículas de plata con Chlorella sp.

Alberto Ricardo Albis Arrieta
Universidad del Atlántico
Fredy Andrés Gonzalez Ortiz
Universidad del Atlántico
Daniel Torrenegra Escorcia
Universidad del Atlántico

Publicado 2021-07-27

Palabras clave

  • Química Verde,
  • Nanopartículas de Plata,
  • Biosíntesis,
  • Microalga

Cómo citar

Albis Arrieta, A. R., Gonzalez Ortiz, F. A., & Torrenegra Escorcia, D. (2021). Biosíntesis de nanopartículas de plata con Chlorella sp. Revista ION, 34(2), 7–15. https://doi.org/10.18273/revion.v34n2-2021001

Resumen

En este trabajo se muestran los resultados de la biosíntesis de nanopartículas de plata mediante el uso de la microalga Chlorella sp, utilizando medios de crecimiento con diferentes concentraciones de glicerol, entre el 5% - 20%, y diferentes condiciones de luz y temperatura. La síntesis de las nanopartículas se estudió utilizando los sobrenadantes y pellets de los cultivos autótrofos, heterótrofos y mixotróficos de la microalga. La presencia de nanopartículas se verificó por espectroscopía ultravioleta – visible y las muestras que mostraron mayor concentración de nanopartículas fueron caracterizadas por microscopía electrónica de barrido. Las condiciones de crecimiento mixotrófico favorecieron la excreción de exopolímeros que mejoraron la reducción de la plata y por ende la formación de nanopartículas. Las nanopartículas obtenidas presentaron forma predominantemente elipsoidal con dimensiones de 108 nm x 156 nm y 87 nm x 123 nm para las reducciones llevadas a cabo con los sobrenadantes de los cultivos mixotróficos con glicerol al 5% y 10%, respectivamente.

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