Articles
Silver nanoparticles functionalized in situ with D-Limonene: effect on antibacterial activity
Julian Echeverry-Chica- Andrea Naranjo-Díaz
- Pedronel Araque-Marín
Published 2020-06-30
Keywords
- Silver Nanoparticles,
- Phase Inversion,
- Bacterial Resistance,
- Minimal Inhibitory Concentration
How to Cite
Echeverry-Chica, J., Naranjo-Díaz, A., & Araque-Marín, P. (2020). Silver nanoparticles functionalized in situ with D-Limonene: effect on antibacterial activity. Revista ION, 33(1), 77–90. https://doi.org/10.18273/revion.v33n1-2020008
Abstract
This study focused on the formulation and characterization of silver nanoparticles (AgNP) functionalized with d-limonene. The nanoparticles were functionalized by phase inversion and the synthesis of the nanoparticles was performed in situ; the particle size was determined by laser diffraction, zeta potential and colloidal optical stability using Multiscan 20 for a period of 24 hours at 37 °C; the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (CMB) of the formulated material on the bacterias Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Klebsiella oxytoca ATCC 700324, Enterococcus casseliflavus ATCC 700327, Escherichia coli BLEE, Pseudomona aeruginosa resistant to carbapenems. The nanoparticles showed colloidal stability at a concentration of 3.93 % d-limonene, 1.6x10-3 % silver ions, 24 % non-ionic adjuvant and 5.88 % ascorbic acid, citric acid/citrate was used as a regulatory system (1:1) 0.48 M for a pH of 4.5. The formulation was classified as a polydispersed system (PD = 0.0851), with a zeta potential of -11.6 mV and average particle size of 81.5 ± 0.9 nm. A particle migration rate of -0.199 ± 0.006 mm h-1 was evidenced, a constant transmission profile, a backscatter profile with variations of 10 %, which represents a stable formulation. The nanoparticles presented an MIC and an MIB of 28 μg mL-1 (5.6x10-2 % limonene, 4.7x10-5 % AgNP) against all the bacteria tested.
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References
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[44] Katz L, Baltz R. Natural product discovery: past, present, and future. J Ind Microbiol Biotechnol. 2016;43(2-3):155-76. https://doi.org/10.1007/s10295-015-1723-5
[2] Ansari M, Khan H, Khan A, Cameotra S, Saquib Q, Musarrat J. Gum arabic capped-silver nanoparticles inhibit biofilm formation by multi-drug resistant strains of Pseudomonas aeruginosa. J. Basic Microbiol. 2014;54(7):688-99. https://doi.org/10.1002/jobm.201300748
[3] Rai M, Deshmukh S, Ingle A, Gade A. Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria. J. Appl. Microbiol. 2012;112(5):841-52. https://doi.org/10.1111/j.1365-2672.2012.05253.x
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[5] Pérez ZC, Torres C, Nuñez M. Antimicrobial Activity and Chemical Composition of Essential Oils from Verbenaceae Species Growing in South America. Molecules. 2018;23(3):544. https://doi.org/10.3390/molecules23030544
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[7] Sheikholeslami S, Mousavi S, Ahmadi AH, Hosseini DS, Mahdi RS. Antibacterial Activity of Silver Nanoparticles and Their Combination with Zataria multiflora Essential Oil and Methanol Extract. Jundishapur J. Microbiol. 2016;9(10):e36070. https://doi.org/10.5812/jjm.36070
[8] Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K. Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity. Spectrochim. Acta, Part A. 2011;79(3):594-98. https://doi.org/10.1016/j.saa.2011.03.040
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[11] Morejón García M. Betalactamasas de espectro extendido. Rev. Cubana Med. 2013;52(4):272-80.
[12] Suarez C, Kattan J, Guzmán A, Villegas M. Mecanismos de resistencia a carbapenems en P. aeruginosa, Acinetobacter y Enterobacteriaceae y estrategias para su prevención y control. Infectio. 2006;10(3):85-93.
[13] Torrenegra M, Pájaro N, Méndez L. Actividad antibacteriana in vitro de aceites esenciales de diferentes especies del género Citrus. Rev. Colomb. Cienc. Quim.-Farm. 2017;46(2):160-75.https://doi.org/10.15446/rcciquifa.v46n2.67934
[14] Shao P, Zhang H, Niu B, Jiang L. Antibacterial activities of R-(+)-Limonene emulsion stabilized by Ulva fasciata polysaccharide for fruit preservation. Int. J. Biol. Macromol. 2018;111:1273-80. https://doi.org/10.1016/j.ijbiomac.2018.01.126
[15] Mitropoulou G, Fitsiou E, Spyridopoulou K, Tiptiri-Kourpeti A, Bardouki H, Vamvakias M, et al. Citrus medica essential oil exhibits significant antimicrobial and antiproliferative activity. LWT-Food Sci. Technol. 2017;84:344-52. https://doi.org/10.1016/j.lwt.2017.05.036
[16] Pekmezovic M, Aleksic I, Barac A, Arsic-Arsenijevic V, Vasiljevic B, Nikodinovic-Runic J, et al. Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. Pathog. Dis. 2016;74(8):ftw102. https://doi.org/10.1093/femspd/ftw102
[17] Montironi I, Cariddi L, Reinoso E. Evaluation of the antimicrobial efficacy of Minthostachys verticillata essential oil and limonene against Streptococcus uberis strains isolated from bovine mastitis. Rev. Argent. Microbiol. 2016;48(3):210-16. https://doi.org/10.1016/j.ram.2016.04.005
[18] Chen G, Lin Y, Lin C, Jen H. Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli. Molecules. 2018;23(4):840. https://doi.org/10.3390/molecules23040840
[19] Lou Z, Chen J, Yu F, Wang H, Kou X, Ma C, et al. The antioxidant, antibacterial, antibiofilm activity of essential oil from Citrus medica L. var. sarcodactylis and its nanoemulsion. LWT. 2017;80:371-7. https://doi.org/10.1016/j.lwt.2017.02.037
[20] Al-Aamri M, Al-Abousi N, Al-Jabri S, Alam T, Khan S. Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in Eastern Oman. J Taibah Univ Med Sci. 2018;13(2):108-12. https://doi.org/10.1016/j.jtumed.2017.12.002
[21] Rudakiya D, Pawar K. Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: in vitro and in silico approaches. 3 Biotech. 2017;7:92. https://doi.org/10.1007/s13205-017-0728-3
[22] Abdel-Aziz M, Shaheen M, El-Nekeety A, Abdel-Wahhab M. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. J. Saudi Chem. Soc. 2014;18(4):356-63. https://doi.org/10.1016/j.jscs.2013.09.011
[23] McQuillan J, Groenaga IH, Stokes E, Shaw A. Silver nanoparticle enhanced silver ion stress response in Escherichia coli K12. Nanotoxicology. 2011;6(8):857-866. https://doi.org/10.3109/17435390.2011.626532
[24] Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine. 2012;8(1):37-45. https://doi.org/10.1016/j.nano.2011.05.007
[25] Vilas V, Philip D, Mathew J. Essential oil mediated synthesis of silver nanocrystals for environmental, anti-microbial and antioxidant applications. Mater. Sci. Eng. 2016;61:429-36. https://doi.org/10.1016/j.msec.2015.12.083
[26] Ramírez LS, Marin Castaño D. Metodologías para evaluar in vitro la actividad antibacteriana de compuestos de origen vegetal. Scientia et Technica. 2009;2(42):263-8.
[27] Herrera ML. Pruebas de sensibilidad antimicrobiana Metodología de laboratorio. Rev. méd. Hosp. Nac. Niños. 1999;34:33-41.
[28] Jiménez N, Cienfuegos A, González G, Higuita L. Medios de cultivo, pruebas de identificación y pruebas de susceptibilidad. Medellín, Colombia: Universidad de Antioquia; 2015.
[29] Picazo JJ. Procedimientos en Microbiología Clínica. Recomendaciones de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. Métodos básicos para el estudio de sensibilidad a los antimicrobianos. España: Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica; 2000.
[30] CLSI M07 - Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 11 Edition. Clinical and Laboratory Standards Institute. 2018.
[31] CLSI. M100 Performance standards for antimicrobial susceptibility testing. 28 Edition. United States: Clinical and Laboratory Standards Institute; 2018.
[32] Scandorieiro S, Camargo L, Lancheros C, Yamada-Ogatta, S, Nakamura, C, Oliveira, A, et al. Synergistic and additive effect of oregano essential oil and biological silver nanoparticles against multidrug-resistant bacterial strains. Front. Microbiol. 2016;7:760. https://doi.org/10.3389/fmicb.2016.00760
[33] Raia M, Paralikara P, Jogeea P, Agarkara G, Inglea A, Deritab M, et al. Synergistic antimicrobial potential of essential oils in combination with nanoparticles: Emerging trends and future perspectives. Int J Pharm. 2017;519(1-2):67-78. https://doi.org/10.1016/j.ijpharm.2017.01.013
[34] Taghizadeh M, Solgi M. The application of essential oils and silver nanoparticles for sterilization of Bermuda grass explants in in vitro culture. Int. J. Hortic. Sci. Technol. 2014;1(2):131–40. https://doi.org/10.22059/IJHST.2014.52784
[35] Khalaf H, Sharoba A, El-Tanahi H, Morsy M. Stability of antimicrobial activity of pullulan edible films incorporated with nanoparticles and essential oils and their impact on turkey deli meat quality. J. Food Dairy Sci. Mansoura Univ. 2013;4(11):557–73. https://doi.org/10.21608/jfds.2013.72104
[36] Bansod S, Bawaskar M, Gade A, Rai M. Development of shampoo, soap and ointment formulated by green synthesized silver nanoparticles functionalized with antimicrobial plants oils in veterinary dermatology: treatment and prevention strategies. IET Nanobiotechnol. 2015;9:165-71. https://doi.org/10.1049/iet-nbt.2014.0042
[37] Cui H, Zhang X, Zhou H, Zhao C, Lin L. Antimicrobial activity and mechanisms of Salvia sclarea essential oil. Bot. Stud. 2015;56(16):2-8. https://doi.org/10.1186/s40529-015-0096-4
[38] Huang D, Xu J, Liu J, Zhang H, Hu Q. Chemical constituents, antibacterial activity and mechanism of action of the essential oil from Cinnamomum cassia bark against four food related bacteria. Microbiology 2014;83:357-65. https://doi.org/10.1134/S0026261714040067
[39] Li C, Yu J. Chemical composition: antimicrobial activity and mechanism of action of essential oil from the leaves of Macleaya cordata (Willd). R. Br. J. Food Saf. 2015;35(2):227-36. https://doi.org/10.1111/jfs.12175
[40] Lakehal S, Meliani A, Benmimoune S, Bensouna S, Benrebiha F, Chaouia, C. Essential oil composition and antimicrobial activity of Artemisia herba– alba Asso grown in Algeria. Med. Chem. (Los Angeles). 2016;6(6):435-9. https://doi.org/10.4172/2161-0444.1000382
[41] Zhang Z, Vriesekoop F, Yuan Q, Liang H, Effects of nisin on the antimicrobial activity of D-limonene and its nanoemulsión. Food Chem. 2014;150:307-12. https://doi.org/10.1016/j.foodchem.2013.10.160
[42] Mohamed A, Mohamed S, Aziza E, Mosaad A. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. J. Saudi Chem. Soc. 2014;18:356-63. https://doi.org/10.1016/j.jscs.2013.09.011
[43] Rhim J, Wang L, Hong S. Preparation and characterization of agar/silver nanoparticles composite films with antimicrobial activity. Food Hydrocolloids. 2013;33(2):327–35. https://doi.org/10.1016/j.foodhyd.2013.04.002.
[44] Katz L, Baltz R. Natural product discovery: past, present, and future. J Ind Microbiol Biotechnol. 2016;43(2-3):155-76. https://doi.org/10.1007/s10295-015-1723-5