Vol. 35 No. 2 (2022): Revista ION
Articles

Validation of a high-performance liquid chromatographic method for the simultaneous determination of diclofenac and ibuprofen in water resources

Juliana Reyes Calle
Politécnico Colombiano Jaime Isaza Cadavid
Alba Nelly Ardila Arias
Grupo de Investigación en Catálisis Ambiental y Energías Renovables (CAMER), Politécnico Colombiano Jaime Isaza Cadavid
Yuleidy Balbin Olarte
Grupo de Investigación en Catálisis Ambiental y Energías Renovables (CAMER), Politécnico Colombiano Jaime Isaza Cadavid
Sergio Andrés Bedoya Urrego
Grupo de Investigación en Catálisis Ambiental y Energías Renovables (CAMER), Politécnico Colombiano Jaime Isaza Cadavid
Erasmo Arriola-Villaseñor
Grupo de Investigación en Catálisis Ambiental y Energías Renovables (CAMER), Politécnico Colombiano Jaime Isaza Cadavid
Eliana Berrio Mesa
Grupo de Investigación en Catálisis Ambiental y Energías Renovables (CAMER), Politécnico Colombiano Jaime Isaza Cadavid

Published 2022-12-05

Keywords

  • Method validation,
  • High-performance liquid chromatography,
  • Diclofenac,
  • Ibuprofen,
  • Water resources

How to Cite

Reyes Calle, J., Ardila Arias, A. N. ., Balbin Olarte, Y. ., Bedoya Urrego, S. A. ., Arriola-Villaseñor, E. ., & Berrio Mesa, E. . (2022). Validation of a high-performance liquid chromatographic method for the simultaneous determination of diclofenac and ibuprofen in water resources. Revista ION, 35(2), 111–125. https://doi.org/10.18273/revion.v35n2-2022009

Abstract

Diclofenac sodium and ibuprofen are highly consumed drugs, used for the relief of common ailments, their poor metabolization means that a large proportion of them become aquifer contaminants, this problem requires urgent attention. The monitoring of these contaminants, through the development and validation of analytical methodologies that allow their quantification in water resources, becomes a necessity to prevent and control them. In this study, a high-performance liquid chromatography method was developed for the simultaneous determination of diclofenac and ibuprofen in water. The validation of the method was carried out with the determination of linearity, limits of quantification and detection, accuracy and precision in terms of repeatability and intermediate precision. For the determination of the performance parameters, the percentage recovery, average, standard deviation, relative standard deviation, coefficient of variation and confidence interval were calculated. In addition, to determine the accuracy and precision, the factorial analysis of variance was applied with a confidence level of 95 % and a significance level of 0.05. The values obtained for all parameters complied with established acceptance criteria, confirming that the method developed is accurate and precise with high levels of reliability.

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References

  1. Eraga SO, Arhewoh MI, Chibuogwu RN, Iwuagwu MA. A comparative UV-HPLC analysis of ten brands of ibuprofen tablets. Asian Pac J Trop Biomed. 2015;5:880–884. doi.org/10.1016/j.apjtb.2015.06.005
  2. Hong S, Xu K, Guo S, Yuan L, Wang H. Development and Validation of a New Simple HPLC Method for the Determination of 3-[4-(2-Methylpropyl)phenyl] Propanoic Acid as an Impurity of Ibuprofen and Ibuprofen Sodium. Chromatographia. 2017;80:1095–1100. doi.org/10.1007/s10337-017-3320-4
  3. Serna-Galvis E, Martínez-Mena YL, Porras J, Torres-Palma RA. Antibióticos de alto consumo en Colombia, excreción en orina y presencia en aguas residuales – una revisión bibliográfica. Ing Y Compet. 2021;24:1–12. doi.org/10.25100/iyc.v24i1.11267
  4. Taheran M, Naghdi M, Brar S, Verma M, Surampalli R. Emerging contaminants: Here Today, There Tomorrow! Environ Nanotechnology, Monit Manag. 2018;10:122-26. doi: 10.1016/j.enmm.2018.05.010
  5. Madera López D, Viloria Soto MF. Contaminantes emergentes en diferentes matrices de aguas y tratamientos alternativos para su eliminación (tesis de pregrado). Montería, Colombia: Universidad de Córdoba; 2020. https://repositorio.unicordoba.edu.co/handle/ucordoba/3572
  6. Caviedes D, Delgado D, Olaya A. Environmental normativity to regulate the presence of residual pharmaceutical products in aquatic environments. Rev Jurídica Piélagus. 2017;16: 21–130. doi.org/10.25054/16576799.1445
  7. United States Pharmacopeia – NF (USP) V 3. USP 38 Diclofenaco Potásico. pp. 3340–3346.
  8. USP-38 I. USP 38 Ibuprofeno. United States Pharmacopeia – NF (USP), V.38, pp. 4207– 4212.
  9. ICH. ICH Harmonised Tripartite Guideline. Validation of analytical procedures: text and methodology Q2(R1). En: International conference on harmonisation of technicalrequirements for registration of pharmaceuticals for human use; 4.
  10. Rivera-utrilla J, Sánchez-polo M, Ferro- García MÁ, Prados-Joya G, Ocampo-Pérez R. Chemosphere Pharmaceuticals as emerging contaminants and their removal from water. A review. Chemosphere. 2013;93:1268–1287. doi.org/10.1016/j.chemosphere.2013.07.059
  11. Eslami A, Amini MM, Yazdanbakhsh AR, Rastkari N, Mohseni-Bandpei A, Nasseri S, et al. Occurrence of non-steroidal antiinflammatory drugs in Tehran source water, municipal and hospital wastewaters , and their ecotoxicological risk assessment. Environ Monit Assess. 2015;187(12):734. doi: 10.1007/s10661-015-4952-1.
  12. Lonappan L, Kaur S, Kumar R, Verma M, Surampalli R. Diclofenac and its transformation products : Environmental occurrence and toxicity - A review. Environ Int. 2016;96:127–138. doi.org/10.1016/j.envint.2016.09.014
  13. Sora IN, Fumagalli D. Fast photocatalytic degradation of pharmaceutical micropollutants and ecotoxicological effects. 2017;24(12):12556–12561. doi.org/10.1007/s11356-016-7640-y
  14. Wilkinson J, Hooda PS, Barker J, Swinden J. Occurrence , fate and transformation of emerging contaminants in water : An overarching review of the fi eld. Environ Pollut. 2017;231:954–970. doi.org/10.1016/j.envpol.2017.08.032
  15. Davarnejad R, Soofi B, Farghadani F, Behfar R. Environmental Technology & Innovation Ibuprofen removal from a medicinal effluent: A review on the various techniques for medicinal effluents treatment. Environ Technol Innov. 2018;11:308–320. doi.org/10.1016/j.eti.2018.06.011
  16. Rodriguez-Narvaez OM, Peralta-Hernandez JM, Goonetilleke A, Bandala E. Treatment technologies for emerging contaminants in water : A review. Chem Eng J. 2017;323:361–380. doi.org/10.1016/j.cej.2017.04.106
  17. Alquadeib BT. Development and validation of a new HPLC analytical method for the determination of diclofenac in tablets. Saudi Pharm J. 2019;27:66–70. doi.org/10.1016/j.jsps.2018.07.020
  18. Payán MR, López MÁB, Fernández-Torres R, Perez-Bernal JL, Callejón Mochón M. HPLC determination of ibuprofen, diclofenac and salicylic acid using hollow fiber-based liquid phase microextraction (HF-LPME). Anal Chim Acta. 2009;653:184–190. doi: 10.1016/j.aca.2009.09.018
  19. Ahmed HM, Elshamy YS, Talaat W, Labib three potential impurities using validated HPLCDAD and HPTLC methods. Microchem J. 2020;153:104505. doi.org/10.1016/j.microc.2019.104505
  20. Klencsár B, Balcaen L, Cuyckens F, Lynen F, Vanhaecke F. Development and validation of a novel quantification approach for gradient elution reversed phase high-performance liquid chromatography coupled to tandem ICPmass spectrometry (RP-HPLC-ICP-MS/MS) and its application to diclofenac and its related compound. Anal Chim Acta. 2017;974:43–53. doi.org/10.1016/j.aca.2017.04.030
  21. Nasir F, Iqbal Z, Khan A, Ahmad L, Shah Y, Khan A, et al. Simultaneous determination of timolol maleate, rosuvastatin calcium and diclofenac sodium in pharmaceuticals and physiological fluids using HPLC-UV. J Chromatogr B Anal Technol Biomed Life Sci. 2011;879:3434–3443. doi: 10.1016/j.jchromb.2011.09.021
  22. Farrar H, Letzig L, Gill M. Validation of a liquid chromatographic method for the determination of ibuprofen in human plasma. J Chromatogr B Anal Technol Biomed Life Sci. 2002;780:341– 348. doi: 10.1016/s1570-0232(02)00543-3
  23. Encarnação T, Aguiar A, Palito C, Pais A, Campos M, Sobral A, et al. Development and validation of a RP-HPLC method for the simultaneous analysis of paracetamol, ibuprofen, olanzapine, and simvastatin during microalgae bioremediation. MethodsX. 2020;7:101083. doi.org/10.1016/j.mex.2020.101083
  24. Yuvali D, Narin I, Soylak M, Yilmaz E. Green synthesis of magnetic carbon nanodot/graphene oxide hybrid material (Fe3O4@Cnanodot@ GO) for magnetic solid phase extraction of ibuprofen in human blood samples prior to HPLC-DAD determination. J Pharm Biomed Anal. 2020;179:113001. doi.org/10.1016/j.jpba.2019.113001.
  25. Aguilar-Romero I, Romero E, Wittich RM, Dillewijn P. Bacterial ecotoxicity and shifts in bacterial communities associated with the removal of ibuprofen, diclofenac and triclosan in biopurification systems. Sci Total Environ. 2020;741:140461. doi: 10.1016/j.scitotenv.2020.140461.
  26. Han Z, Lu L, Wang L, Yan Z, Wang X. Development and Validation of an HPLC Method for Simultaneous Determination of Ibuprofen and 17 Related Compounds. Chromatographia. 2017;80:1353–1360. doi.org/10.1007/s10337-017-3358-3