Vol. 36 No. 3 (2023): Revista ION
Articles

Use of the spectrophotometric method for cell quantification of marine microalgae for use in aquaculture

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  • RUTH MILAGROS ALEJOS CABRERA,
  • Gheraldine Abegail Ynga Huamán,
  • Wilmer Alexis Gaspar Reyes
RUTH MILAGROS ALEJOS CABRERA
Instituto del Mar del Perú
Gheraldine Abegail Ynga Huamán
Instituto del Mar del Perú
Wilmer Alexis Gaspar Reyes
Instituto del Mar del Perú
DOI: https://doi.org/10.18273/revion.v36n3-2023007

Published 2023-12-12

Keywords

  • Absorbance,
  • Optical density,
  • Spectrophotometry,
  • Microalgae

How to Cite

ALEJOS CABRERA, R. M., Ynga Huamán, G. A., & Gaspar Reyes, W. A. (2023). Use of the spectrophotometric method for cell quantification of marine microalgae for use in aquaculture. Revista ION, 36(3), 75–84. https://doi.org/10.18273/revion.v36n3-2023007

Copyright (c) 2023 RUTH MILAGROS ALEJOS CABRERA, Gheraldine Abegail Ynga Huamán, Wilmer Alexis Gaspar Reyes

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

In the present study, we proposed to develop a predictive model that allows quantifying the cell density of microalgae from absorbance. The specific wavelength for the maximum absorbance of two species of marine microalgae of importance in aquaculture was determined: Isochrysis galbana (680 nm) and Chaetoceros calcitrans (676 nm). Subsequently, the predictive model was generated through the construction of a calibration curve, five levels were used for each species and it was carried out quintuplicate. The concentration range for C. calcitrans was 0,8–4,3 x 106 cells/ml (0,8; 1,7; 2,5; 3,4 and 4,3 x 106 cells/ml) and for I. galbana it was 1,6–7,8 x 106 cells/ml (1,6; 3,2; 4,7; 6,3 and 7,8 x 106 cell/ml). As a result, the following equations were obtained: y = 0,0004 + 0,0581*(AbsI. galbana) and y = 0,0065 + 0,1001*(AbsC. calcitrans); where the coefficients of determination (R2) were high 0,991 and 0,981 for I. galbana and C. calcitrans, respectively; hence, indicates that absorbance and cell density are closely related to each other. Therefore, the linear model equation allows determination of cell density as a function of absorbance.

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