Nanopartículas de plata funcionalizadas in situ con D-limoneno: efecto en la actividad antibacteriana

Resumen

Este estudio se centró en la formulación y caracterización de nanopartículas de plata (AgNP) funcionalizadas con d-limoneno. Las nanopartículas se funcionalizaron por inversión de fase y la síntesis de las nanopartículas se realizó in situ; se determinó el tamaño de partícula por difracción laser, potencial zeta y estabilidad coloidal óptica utilizando Multiscan 20 por un periodo de 24 horas a 37 °C; se determinó la concentración mínima inhibitorio (CMI) y la concentración mínima bactericida (CMB) del material formulado sobre las bacterias Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Klebsiella oxytoca ATCC 700324, Enterococcus casseliflavus ATCC 700327, Escherichia coli BLEE+, Pseudomona aeruginosa resistente a carbapenémicos. Las nanopartículas presentaron estabilidad coloidal a una concentración de d-limoneno del 3,93 %, iones plata al 1,61x0-3 %, coadyuvante no iónico al 24 % y ácido ascórbico al 5,88 %; cómo sistema regulador se utilizó ácido cítrico/citrato (1:1) 0,48M para un pH de 4,5. La formulación se clasificó como un sistema polidisperso (PD = 0,0851), con una potencial zeta de -11,6 mV y tamaño promedio de partícula de 81,5 ± 0,9 nm. Se evidenció una velocidad de migración de partícula de -0,199 ± 0,006 mm h-1, un perfil de transmisión constante y perfil de retrodispersión con variaciones del 10 %, lo que representa una formulación estable. Las nanopartículas presentaron una CMI y una CMB de 28 μg mL-1 (5,6x10-2 % d-limoneno y 4,7x10-5 % AgNP) contra todas las bacterias probadas.

Palabras clave: Nanopartículas de Plata, Inversión de Fases, Resistencia Bacteriana, Concentración Mínima Inhibitoria

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Publicado
2020-06-30
Sección
Artículos