Vol. 36 No. 1 (2023): Revista ION
Articles

Phytochemical characterization of Calendula officinalis extracts

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  • María Julia López Vallejos,
  • Laura Beatriz Huber,
  • Patricia Alejandra Vaculik,
  • Roque Oscar Rosende,
  • Laura Analía Lozina
María Julia López Vallejos
Docente
Laura Beatriz Huber
Universidad Nacional del Nordeste
Patricia Alejandra Vaculik
Universidad Nacional del Nordeste
Roque Oscar Rosende
Universidad Nacional del Nordeste
Laura Analía Lozina
Universidad Nacional del Nordeste
DOI: https://doi.org/10.18273/revion.v36n1-2023007

Published 2023-02-28

Keywords

  • Chemical characterization,
  • Natural products,
  • Organoleptic properties

How to Cite

López Vallejos, M. J., Huber, L. B., Vaculik, P. A. ., Rosende, R. O. ., & Lozina , L. A. . (2023). Phytochemical characterization of Calendula officinalis extracts. Revista ION, 36(1), 91–99. https://doi.org/10.18273/revion.v36n1-2023007

Copyright (c) 2023 María Julia López Vallejos, Laura Beatriz Huber, Patricia Alejandra Vaculik, Roque Oscar Rosende, Laura Analía Lozina

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Calendula officinalis is a plant known for its therapeutic qualities, pharmacological studies carried out with extracts showed antimicrobial, antioxidant, anti-inflammatory activities, etc. The presence of flavoniodes and phenols are responsible for these activities. The objective of this work was to obtain Calendula officinalis extracts with different solvents and determine their phytochemical characterization. Flower extracts were obtained and subjected to extraction by simple maceration with 96 ° alcohol,
methanol and a hydroalcoholic solution, for 24 h with permanent stirring. Organoleptic and phytochemical characteristics, antioxidant activity were established by means of the Oxidation index and determination of phenolic compounds by the Folin-Cicoalteu method. PH measurements, chromatographic analysis and phytochemical analysis were carried out to show the presence of secondary metabolites. The hydroalcoholic extract presented better organoleptic characteristics, good performance and excellent oxidation index. The quantification of phenols obtained good standards in the three extracts, the hydroalcholic being higher, the pHs were presented in a range of 6. The chromatography determined that the run solvents were effective for the separation of compounds, the reference of phenols and flavonoids used as quercetin, rutin, and chlorogenic acid were positive. The phytochemical march determined the presence of flavonoids, phenols, alkaloids, glycosides and anthraquinones in the secondary metabolites.

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