Artigos
Potencial bioquímico de metano da Chlorella vulgaris: Influência da hidrólise térmica
Publicado 2019-01-16
Palavras-chave
- microalga,
- pré-tratamento,
- digestão anaeróbica,
- produção de metano
Como Citar
Cerón-Vivas, A., Acosta, J. P., Alvear, L. V., & Gamarra, Y. (2019). Potencial bioquímico de metano da Chlorella vulgaris: Influência da hidrólise térmica. REVISTA ION, 31(2). https://doi.org/10.18273/revion.v31n2-2018002
Resumo
A investigação sobre a transformação de biomassa em biocombustíveis tem aumentado nos últimos anos. A digestão anaeróbia é um processo biológico em que o produto principal obtido é o biogás. Biomassa de microalgas produzido em plantas de tratamento de águas residuais pode ser utilizado como um substrato para a digestão anaeróbica. No entanto, para melhorar a produtividade de metano, que é necessário para quebrar a parede celular de microalgas utilizando pré-tratamentos. Assim, este estudo avaliou a influência da hidrólise térmica no potencial bioquímico de metano da Chlorella vulgaris. A qualidade do inóculo usado foi verificada avaliando a atividade de grupos tróficos (hidrolítica, acidogênica e metanogênica). A influência da hidrólise térmica na produção de metano foi avaliada aplicando o modelo modificado de Gompertz. Esse modelo foi capaz de prever a produtividade final para microalgas colhidas por centrifugação e para submetido a hidrólise térmica. A taxa máxima de produtividade aumentou de 18,4 ± 1,0 ml.g-1VS.d-1 a 29,0 ± 3,1 ml.g-1VS.d-1, quando a hidrólise térmica foi aplicada às microalgas. Isso pode ser explicado devido ao rompimento da parede celular, resultando no aumento da matéria orgânica solúvel e produção do gás metano. Os resultados sugerem que a hidrólise térmica da C. vulgaris pode ser utilizado como pré-tratamento para melhorar o desempenho da geração de metano na digestão anaeróbica.
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Referências
[1] Aslan S, Kapdan IK. Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae. Ecol Eng [Internet]. 2006 Nov [cited 2014 Jul 15];28(1):64–70. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0925857406000759.
[2] Cai T, Park SY, Li Y. Nutrient recovery from wastewater streams by microalgae: Status and prospects. Renew Sustain Energy Rev [Internet]. 2013 Mar [cited 2014 Jul 14];19:360–9. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1364032112006429.
[3] Safi C, Zebib B, Merah O, Pontalier P-Y, Vaca-Garcia C. Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renew Sustain Energy Rev. 2014;35:265–78.
[4] Singh J, Gu S. Commercialization potential of microalgae for biofuels production. Renew Sustain Energy Rev. 2010;14(9):2596–610.
[5] Chisti Y. Biodiesel from microalgae beats bioethanol. Trends Biotechnol. 2008;26:126–31.
[6] Mubarak M, Shaija A, Suchithra T V. Review on the extraction of lipid from microalgae for biodiesel production. Algal Res. 2015;7:117–23.
[7] Harun R, Danquah MK. Enzymatic hydrolysis of microalgal biomass for bioethanol production. Chem Eng J. 2011;168(3):1079–84.
[8] Lundquist IW. A realistic technology and engineering assessment of algae biofuel production. 2010.
[9] Alzate ME, Muñoz R, Rogalla F, Fdz-Polanco F, Pérez-Elvira SI. Biochemical methane potential of microalgae biomass after lipid extraction. Chem Eng J. 2014;243:405–10.
[10] Prajapati SK, Kaushik P, Malik A, Vijay VK. Phycoremediation coupled production of algal biomass, harvesting and anaerobic digestion: possibilities and challenges. Biotechnol Adv. 2013;31(8):1408–25.
[11] Prajapati SK, Malik A, Vijay VK. Comparative evaluation of biomass production and bioenergy generation potential of Chlorella spp. through anaerobic digestion. Appl Energy. 2014;114:790–7.
[12] Alzate ME, Muñoz R, Rogalla F, Fdz-Polanco F, Pérez-elvira SI. Biochemical methane potential of microalgae : Influence of substrate to inoculum ratio , biomass concentration and pretreatment. Bioresour Technol. 2012;123:488–94.
[13] González-Fernández C, Sialve B, Bernet N, Steyer JP. Comparison of ultrasound and thermal pretreatment of Scenedesmus biomass on methane production. Bioresour Technol. 2012;110:610–6.
[14] Passos F, Solé M, García J, Ferrer I. Biogas production from microalgae grown in wastewater: Effect of microwave pretreatment. Appl Energy. 2013;108:168–75.
[15] Klassen V, Blifernez-klassen O, Wobbe L, Schlüter A, Kruse O, Mussgnug JH. Efficiency and biotechnological aspects of biogas production from microalgal substrates. J Biotechnol. 2016;234:7–26.
[16] Bischoff H., Bold HC. Phycological studies IV. In: Some soil algae from Enchanted Rock and related algal species. Austin: University of Texas; 1963. p. 1–95.
[17] APHA, AWWA, WEF. Standard Methods for the Examination of Water and Wastewater. 22th editi. Washington DC.; 2012.
[18] Field J. Parametros operativos del manto de lodos anaeróbicos de flujo ascendente. In: Manual de Arranque y operación de flujo ascendente con manto de lodos - UASB. Cali: Universidad del Valle, CVC. Universidad Agrícola de Wageningen.; 1987. p. 270.
[19] Miller GL. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal Chem. 1953;31(3):426–8.
[20] Dubois M, Pilles K, Hamilton J, Smith F. Colorimetric method for determination of sugar and related substances. Anal Chem. 1956;28:350–6.
[21] Poirrier González P. Hidrólisis y acidificación psicrófila de moléculas complejas en sistemas anaerobios. Santiago de Compostela, España: Universidad de Santiago de Compostela; 2005.
[22] Zwietering MH, Jongenburger I, Rombouts FM, Van’t Riet K. Modeling of the bacterial growth curve. Appl Environ Microbiol. 1990;56(6):1875–81.
[23] Jiunn-Jyi L, Yu-You L, Noike T. Influences of pH and moisture content on the methane production in high-solids sludge digestion. Water Res. 1997;31(6):1518–24.
[24] Castro Echavez FL, Fernández Acosta NM, Delgado Chávez MJ. Disminución de la DQO en aguas de formación utilizando cepas bacterianas. Rev Técnica la Fac Ing Univ del Zulia. 2008;31(3):246–55.
[25] Herrera Soracá DM, Niño Bonilla DA. Evaluación del potencial producción de biogas a partir de aguas residuales provenientes de la industria palmera mediante la digestión anaerobia. Universidad Industrial de Santander; 2011.
[26]Instituto para la Diversificación y Ahorro de la Energía-IDAE. Biomasa:Digestores Anaerobios. [Internet]. Madrid, España: Instituto para la Diversificación y Ahorro de la Energía; 2007.48 p. Available from: http://www.idae.es/uploads/documentos/documentos_10737_Biomasa_digestores_07_a996b846.pdf.
[27] Quintero M, Castro L, Ortiz C, Guzmán C, Escalante H. Enhancement of starting up anaerobic digestion of lignocellulosic substrate: Fique’s bagasse as an example. Bioresour Technol. 2012;108:8–13.
[28] Regueiro L, Veiga P, Figueroa M, Alonso-Gutierrez J, Stams AJM, Lema JM, et al. Relationship between microbial activity and microbial community structure in six full-scale anaerobic digesters. Microbiol Res. 2012;167(10):581–9.
[29] Díaz-Báez MC, Vargas SLE, Pérez FM. Digestión anaerobia: una aproximacion a la tecnología. First. Bogotá D.C., Colombia: Universidad Nacional de Colombia; 2002. 168 p.
[30] Effebi KR, Baya T, Jupsin H, Vasel JL. Acidogenic and Methanogenic activities in Anaerobic Ponds. 2011;2(12):1–4.
[31] Soto M, Méndez R, Lema JM. Methanogenic and non-methanogenic activity tests. Theoretical basis and experimental set up. Water Res [Internet]. 1993 Aug [cited 2015 Sep 7];27(8):1361–76. Available from: http://www.sciencedirect.com/science/article/pii/0043135493902246.
[32] Passos F, Ferrer I. Influence of hydrothermal pretreatment on microalgal biomass anaerobic digestion and bioenergy production. Water Res [Internet]. 2014;68:364–73. Available from: http://dx.doi.org/10.1016/j.watres.2014.10.015.
[33] Cho S, Park S, Seon J, Yu J, Lee T. Evaluation of thermal, ultrasonic and alkali pretreatments on mixed-microalgal biomass to enhance anaerobic methane production. Bioresour Technol [Internet]. 2013 Sep [cited 2014 Jul 17];143:330–6. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23811066.
[34] Passos F, Ferrer I. Microalgae Conversion to Biogas: Thermal Pretreatment Contribution on Net Energy Production. Environ Sci Technol [Internet]. 2014 Jun 17;48(12):7171–8. Available from: https://doi.org/10.1021/es500982v.
[2] Cai T, Park SY, Li Y. Nutrient recovery from wastewater streams by microalgae: Status and prospects. Renew Sustain Energy Rev [Internet]. 2013 Mar [cited 2014 Jul 14];19:360–9. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1364032112006429.
[3] Safi C, Zebib B, Merah O, Pontalier P-Y, Vaca-Garcia C. Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renew Sustain Energy Rev. 2014;35:265–78.
[4] Singh J, Gu S. Commercialization potential of microalgae for biofuels production. Renew Sustain Energy Rev. 2010;14(9):2596–610.
[5] Chisti Y. Biodiesel from microalgae beats bioethanol. Trends Biotechnol. 2008;26:126–31.
[6] Mubarak M, Shaija A, Suchithra T V. Review on the extraction of lipid from microalgae for biodiesel production. Algal Res. 2015;7:117–23.
[7] Harun R, Danquah MK. Enzymatic hydrolysis of microalgal biomass for bioethanol production. Chem Eng J. 2011;168(3):1079–84.
[8] Lundquist IW. A realistic technology and engineering assessment of algae biofuel production. 2010.
[9] Alzate ME, Muñoz R, Rogalla F, Fdz-Polanco F, Pérez-Elvira SI. Biochemical methane potential of microalgae biomass after lipid extraction. Chem Eng J. 2014;243:405–10.
[10] Prajapati SK, Kaushik P, Malik A, Vijay VK. Phycoremediation coupled production of algal biomass, harvesting and anaerobic digestion: possibilities and challenges. Biotechnol Adv. 2013;31(8):1408–25.
[11] Prajapati SK, Malik A, Vijay VK. Comparative evaluation of biomass production and bioenergy generation potential of Chlorella spp. through anaerobic digestion. Appl Energy. 2014;114:790–7.
[12] Alzate ME, Muñoz R, Rogalla F, Fdz-Polanco F, Pérez-elvira SI. Biochemical methane potential of microalgae : Influence of substrate to inoculum ratio , biomass concentration and pretreatment. Bioresour Technol. 2012;123:488–94.
[13] González-Fernández C, Sialve B, Bernet N, Steyer JP. Comparison of ultrasound and thermal pretreatment of Scenedesmus biomass on methane production. Bioresour Technol. 2012;110:610–6.
[14] Passos F, Solé M, García J, Ferrer I. Biogas production from microalgae grown in wastewater: Effect of microwave pretreatment. Appl Energy. 2013;108:168–75.
[15] Klassen V, Blifernez-klassen O, Wobbe L, Schlüter A, Kruse O, Mussgnug JH. Efficiency and biotechnological aspects of biogas production from microalgal substrates. J Biotechnol. 2016;234:7–26.
[16] Bischoff H., Bold HC. Phycological studies IV. In: Some soil algae from Enchanted Rock and related algal species. Austin: University of Texas; 1963. p. 1–95.
[17] APHA, AWWA, WEF. Standard Methods for the Examination of Water and Wastewater. 22th editi. Washington DC.; 2012.
[18] Field J. Parametros operativos del manto de lodos anaeróbicos de flujo ascendente. In: Manual de Arranque y operación de flujo ascendente con manto de lodos - UASB. Cali: Universidad del Valle, CVC. Universidad Agrícola de Wageningen.; 1987. p. 270.
[19] Miller GL. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal Chem. 1953;31(3):426–8.
[20] Dubois M, Pilles K, Hamilton J, Smith F. Colorimetric method for determination of sugar and related substances. Anal Chem. 1956;28:350–6.
[21] Poirrier González P. Hidrólisis y acidificación psicrófila de moléculas complejas en sistemas anaerobios. Santiago de Compostela, España: Universidad de Santiago de Compostela; 2005.
[22] Zwietering MH, Jongenburger I, Rombouts FM, Van’t Riet K. Modeling of the bacterial growth curve. Appl Environ Microbiol. 1990;56(6):1875–81.
[23] Jiunn-Jyi L, Yu-You L, Noike T. Influences of pH and moisture content on the methane production in high-solids sludge digestion. Water Res. 1997;31(6):1518–24.
[24] Castro Echavez FL, Fernández Acosta NM, Delgado Chávez MJ. Disminución de la DQO en aguas de formación utilizando cepas bacterianas. Rev Técnica la Fac Ing Univ del Zulia. 2008;31(3):246–55.
[25] Herrera Soracá DM, Niño Bonilla DA. Evaluación del potencial producción de biogas a partir de aguas residuales provenientes de la industria palmera mediante la digestión anaerobia. Universidad Industrial de Santander; 2011.
[26]Instituto para la Diversificación y Ahorro de la Energía-IDAE. Biomasa:Digestores Anaerobios. [Internet]. Madrid, España: Instituto para la Diversificación y Ahorro de la Energía; 2007.48 p. Available from: http://www.idae.es/uploads/documentos/documentos_10737_Biomasa_digestores_07_a996b846.pdf.
[27] Quintero M, Castro L, Ortiz C, Guzmán C, Escalante H. Enhancement of starting up anaerobic digestion of lignocellulosic substrate: Fique’s bagasse as an example. Bioresour Technol. 2012;108:8–13.
[28] Regueiro L, Veiga P, Figueroa M, Alonso-Gutierrez J, Stams AJM, Lema JM, et al. Relationship between microbial activity and microbial community structure in six full-scale anaerobic digesters. Microbiol Res. 2012;167(10):581–9.
[29] Díaz-Báez MC, Vargas SLE, Pérez FM. Digestión anaerobia: una aproximacion a la tecnología. First. Bogotá D.C., Colombia: Universidad Nacional de Colombia; 2002. 168 p.
[30] Effebi KR, Baya T, Jupsin H, Vasel JL. Acidogenic and Methanogenic activities in Anaerobic Ponds. 2011;2(12):1–4.
[31] Soto M, Méndez R, Lema JM. Methanogenic and non-methanogenic activity tests. Theoretical basis and experimental set up. Water Res [Internet]. 1993 Aug [cited 2015 Sep 7];27(8):1361–76. Available from: http://www.sciencedirect.com/science/article/pii/0043135493902246.
[32] Passos F, Ferrer I. Influence of hydrothermal pretreatment on microalgal biomass anaerobic digestion and bioenergy production. Water Res [Internet]. 2014;68:364–73. Available from: http://dx.doi.org/10.1016/j.watres.2014.10.015.
[33] Cho S, Park S, Seon J, Yu J, Lee T. Evaluation of thermal, ultrasonic and alkali pretreatments on mixed-microalgal biomass to enhance anaerobic methane production. Bioresour Technol [Internet]. 2013 Sep [cited 2014 Jul 17];143:330–6. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23811066.
[34] Passos F, Ferrer I. Microalgae Conversion to Biogas: Thermal Pretreatment Contribution on Net Energy Production. Environ Sci Technol [Internet]. 2014 Jun 17;48(12):7171–8. Available from: https://doi.org/10.1021/es500982v.