Vol. 37 No. 3 (2024): Revista ION
Articles

Sweet potato peel peroxidasemodified graphene oxide electrodes for detection of hydrogen peroxide via electrochemical sensing

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  • John J. Castillo ,
  • Miguel Ángel Vega
John J. Castillo
Universidad Industrial de Santander
Miguel Ángel Vega
Universidad Industrial de Santander
Graphical abstract
DOI: https://doi.org/10.18273/revion.v37n3-2024004

Published 2024-12-09

Keywords

  • Sweet potato peel,
  • Peroxidase,
  • Cyclic voltammetry,
  • Sensing,
  • Hydrogen peroxide

How to Cite

Castillo , J. J., & Vega, M. Ángel. (2024). Sweet potato peel peroxidasemodified graphene oxide electrodes for detection of hydrogen peroxide via electrochemical sensing. Revista ION, 37(3), 43–55. https://doi.org/10.18273/revion.v37n3-2024004

Copyright (c) 2024 John J. Castillo, Miguel Ángel Vega

Creative Commons License

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

Abstract

The development of efficient and sensitive detection methods for hydrogen peroxide (H2O2) is crucial for various applications in biology, medicine, and environmental monitoring. Here, we present a novel approach utilizing batata peel peroxidase (BPP) extract-modified screen-printed graphene oxide electrodes (SPGOE) for the electrochemical sensing of H2O2. The BPP was characterized as having a specific activity of 478 U mg-1, an optimum pH of 8.0 and a thermostability at 60°C with a Kinact of 7.02x10-3 min-1. In this study, we systematically investigate the fabrication process of the peroxidase batata-modified SPGOE and characterize their electrochemical performance using cyclic voltammetry technique. The BPP-SPGOE demonstrates outstanding electrocatalytic performance for the reduction of H₂O₂, showing a linear response across the 250 μM to 5 mM concentration range and a detection limit of 4.6 mM. This novel sensor, created by incorporating BPP onto the GO electrode, offers a promising electrochemical detection system for measuring H₂O₂ in real-world samples, which has significant biomedical and environmental applications. Overall, this study presents a versatile and efficient strategy for electrochemical sensing of H2O2 using BPP-SPGOE, paving the way for advanced analytical methodologies with broad applications in biology and beyond.

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