Vol. 37 Núm. 3 (2024): Revista ION
Artículos

Electrodos de óxido de grafeno modificados con peroxidasa de cáscara de batata para la detección de peróxido de hidrógeno mediante sensado electroquímico

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  • John J. Castillo,
  • Miguel Ángel Vega
John J. Castillo
Universidad Industrial de Santander
Miguel Ángel Vega
Universidad Industrial de Santander
Resumen gráfico
DOI: https://doi.org/10.18273/revion.v37n3-2024004

Publicado 2024-12-09

Palabras clave

  • Cáscara de batata,
  • Peroxidasa,
  • Voltamperometría cíclica,
  • Detección,
  • Peróxido de hidrógeno

Cómo citar

Castillo, J. J., & Vega, M. Ángel. (2024). Electrodos de óxido de grafeno modificados con peroxidasa de cáscara de batata para la detección de peróxido de hidrógeno mediante sensado electroquímico. Revista ION, 37(3), 43–55. https://doi.org/10.18273/revion.v37n3-2024004

Derechos de autor 2024 John J. Castillo, Miguel Ángel Vega

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-SinDerivadas 4.0.

Resumen

El desarrollo de métodos de detección eficientes y sensibles para el peróxido de hidrógeno (H2O2) es crucial para diversas aplicaciones en biología, medicina y monitoreo ambiental. Aquí, presentamos un enfoque novedoso que utiliza electrodos de óxido de grafeno (OG) impresos en pantalla modificados con extracto de peroxidasa de cáscara de batata (PCB) para la detección electroquímica de H2O2. La BPP se caracterizó por tener una actividad específica de 478 Umg-1, un pH óptimo de 8.0 y una termoestabilidad a 60°C con un Kinact de 7.02x10-3min-1. En este estudio, investigamos sistemáticamente el proceso de fabricación de los electrodos modificados con PCB y caracterizamos su rendimiento electroquímico utilizando la técnica de voltamperometría cíclica (VC). El PCB-ESOG demuestra un rendimiento electrocatalítico sobresaliente para la reducción de H2O2, mostrando una respuesta lineal en el rango de concentración de 250 μM a 5 mM y un límite de detección de 4.6 mM. Este novedoso sensor, creado mediante la incorporación de BPP en el electrodo de OG, ofrece un sistema de detección electroquímica prometedor para medir H2O2 en muestras reales, lo cual tiene importantes aplicaciones biomédicas y ambientales. En general, este estudio presenta una estrategia versátil y eficiente para la detección electroquímica de H2O2 utilizando BPP-ESOG, explorando el camino para metodologías analíticas avanzadas con amplias aplicaciones en biología.

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