v. 37 n. 3 (2024): Revista ION
Artigos

Eletrodos de óxido de grafeno modificados com peroxidase da casca de batata-doce para a detecção de peróxido de hidrogênio via sensoramento eletroquímico

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  • John J. Castillo,
  • Miguel Ángel Vega
John J. Castillo
Universidad Industrial de Santander
Miguel Ángel Vega
Universidad Industrial de Santander
Resumo gráfico
DOI: https://doi.org/10.18273/revion.v37n3-2024004

Publicado 2024-12-09

Palavras-chave

  • Casca de batata-doce,
  • Peroxidase,
  • Voltametria cíclica,
  • Sensoramento,
  • Peróxido de hidrogênio

Como Citar

Castillo, J. J., & Vega, M. Ángel. (2024). Eletrodos de óxido de grafeno modificados com peroxidase da casca de batata-doce para a detecção de peróxido de hidrogênio via sensoramento eletroquímico. REVISTA ION, 37(3), 43–55. https://doi.org/10.18273/revion.v37n3-2024004

Copyright (c) 2024 John J. Castillo, Miguel Ángel Vega

Creative Commons License
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NoDerivatives 4.0 International License.

Resumo

O desenvolvimento de métodos de detecção eficientes e sensíveis para o peróxido de hidrogênio (H2O2) é crucial para várias aplicações em biologia, medicina e monitorização ambiental. Aqui, apresentamos uma abordagem inovadora utilizando eletrodos de óxido de grafeno impressos em tela (SPGOE) modificados com extrato de peroxidase da casca de batata (BPP) para a detecção eletroquímica de H2O2. A BPP foi caracterizada como tendo uma atividade específica de 478 U mg-1, um pH ótimo de 8,0 e uma termoestabilidade a 60°C com um Kinact de 7,02x10-3 min-1. Neste estudo, investigamos sistematicamente o processo de fabricação do SPGOE modificado com peroxidase de batata e caracterizamos seu desempenho eletroquímico utilizando a técnica de voltametria cíclica. O BPP-SPGOE demonstra um desempenho eletrocatalítico excepcional para a redução de H2O2, mostrando uma resposta linear na faixa de concentração de 250 μM a 5 mM e um limite de detecção de 4,6 mM. Este sensor inovador, criado pela incorporação de BPP no eletrodo de GO, oferece um sistema promissor de detecção eletroquímica para medir H2O2 em amostras do mundo real, com aplicações significativas em biomedicina e meio ambiente. No geral, este estudo apresenta uma estratégia versátil e eficiente para a detecção eletroquímica de H2O2 utilizando BPP-SPGOE, abrindo caminho para metodologias analíticas avançadas com amplas aplicações em biologia e além.

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