Artículos
Publicado 2005-06-01
Palabras clave
- Dielectroforésis,
- levaduras,
- manipulación de micropartículas,
- circuitos integrados
Cómo citar
Fernández Morales, F. H., Duarte, J. E., & Samitier Martí, J. (2005). Potencial de los campos eléctricos para la manipulación de microorganismos. Revista UIS Ingenierías, 4(1), 53–63. Recuperado a partir de https://revistas.uis.edu.co/index.php/revistauisingenierias/article/view/2125
Resumen
En el presente artículo se ilustra el potencial que tienen los campos eléctricos no uniformes parala manipulación de micropartículas. Para ello se presentan los principios físicos que rigen elmovimiento de partículas eléctricamente neutras cuando son sometidas a campos eléctricosinhomogéneos. generandofenómenos tales como la dielectroforésis común (DE?). dielectroforésisde onda viajera (TWD) y la electrorotación (ROT). Además se presentan algunos resultados de laverificación experimental de una microestructura en espiral. fabricada en tecnología CMOS.cuya prueba se hizo con microesferas de poliestireno de 6 mm de diámetro. Por último se ilustrala manipulación de levaduras Saccharomyces cerevisiae mediante electrodos de platino queinducen en ellas elfenómeno de dielectroforésis común.
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Referencias
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Nishíoka M., Katsura S.,HíranoK and Mízuno A. 1997. Evaluations of cell characteristics by step-wíse orientationa1 rotationn singop to electrostaticrni cromanipulation. lEEE Trans. on lnd. App 133, 1381-1388.
Po WH. A.. 1951. The motion and precipitation of suspend oids in divergent electric fields. J. of Appl. Phys. 22, 869-871.
Quinn C. M., Archer G. P., Betts W. B. and O'Neill J. G.. 1996. Dose-dependent dielectrophoretic response of Cryptosporodiumoocyst streated whit ozone. Letters in Applied Microbiology 22, 224-228.
Lee S-W, Choi J-H. and Kim Y-K. .1995. Design of a biological cell fusion device. Transducers '95-Eurosensors IX. 377-380.
Gascoyue P. RC., Wang X-B., Huang Y. and Becker F. F.. 1997. Dielectrophoretic separation of cancer cells from blood. lEEE Trans. on lnd. Appl. 33(3), 670-678.
Petlrig R, Huang Y. Wang X-B. and Burtl P. H.. 1992. Positive and negative dielectrophoretic collection of colloidal partides using interdigitated castellatedr nicro electrodes. l Phys. D: Appl. Phys. 24, 881-888.
Fubr G. and Wagner B.. 1994. Electric field mediated cellmanipulation in híghly conductive culture media. Micro-system technologies'9 4I4th lnternational Conference on Micro, Electro, Opto, Mechanical Systems and Components. Berlin, Germany. 407-416.
Washizu M.. 1990. Electrostatic manipu1atiou of biological objects. Jouma1 of Electrostatics 25, 109-123.
Wang X-B., Huang Y., Gascoyne P. R. C. and Becker F. F.. 1997. Dielectrophoretic manipulation of particles. IEEE Trans. onlnd. App!. 33(3), 660-669.
Barrie G., Paul R, Kaler K. and Jones T. B.. 1995. Low-frequency micromotion of DEP-Levitated bioparticles, II: Spectral and stability analysis. Joumal of Colloid and Interface Science 175, 97-107.
Fuln G., Müller T., Schnelle T., Hagedorn R, Voigt A. and FiedIerS.. 1994. Radio-frequeucy microtools for partic1e and livingcel! manipnlation. Natur wissens chaften 81, 528-535.
Cornmings E.. 2003. Streaming dielectrophoresis for contiunous: fIow microfiuidic devices. En: IEEE in Medicine and Biology Magazine 22, 75-84.
Park J. and Lakes R.. 1992. Biomaterials: An introductiou. 2nd Editiou. Ed. Pleunm Press. New York
Panl R, Kaler K. V. L. S. and Jones T. B.. 1993. An onequilibrinm statistical mechanical calculation of the surface conductance of the electrical donble layer of biological cel!sandits application to dielectrophoresis. The Jonmal of Physical Chemistry 97 (18), 4745-4755.
Pohl H. A.. 1958. Some effects of nonn niforru fields on dielectrics. J. App!. Phys. 29, 1182-1188.
Pohl H. A. and Pethig R ..1977. Dielectric measurements using non-uniform electric field (dielectro phoretic) effects. 1. Phys. E: Sci. Instruru. lO, 190-193. Corrigendnm 883.
Mü1ler T., Gerardiuo A., Schnelle T., Shírley S. G., Fuhr G., De Gasperis G., Leo Ili Rand Bordo Ili F.. 1995. High-frequency electric-field trap for micron and submi cronparticles. llN novo Cimeuto 17 D. 425-432.
Talary M. S., Burt J. P. H., Tame J. A. and Pethig R.. 1996. Electro manipulation and separatiou of cell susing travelling electric fields. J. Phys. D: Appl. Phys. 29, 2198-2203.
Stephens M, Talary M., Pethig R., Bumett A. Y Milis K.. 1996. The dielectro phoresi seurichment of CD 34+cells from peripheral blood stem cell harvests. Bone Marrow Transplantation 18, 777-782
Pohl H. A. 1978. Dielectrophoresis: The behavior of neutral matterin non unifor ruelectric fields. Ed. Cambridge University Press. London.
Fuhr G. and Shirley S. G.. 1998. Biological application of microstructures. Topics in curreut chemistry 194, 83-116.
Fuhr G., Schnelle T., Müller T., Glasse,. H., Lisec T. aud Waguer B.. 1995. Positioning and manipulation of cells and microparticles using miniatu.lized electrie field trapsaud travelliug waves. Seusors and materials 7, 131-146.
Hughes M. P., Waug X-B., Becker F. F., Gascoyne P-R. C. aud Pethig R 1994. Computer-aided analyses of electric fields used in electro rotatiou studies. J. Phys. D: Appl. Phys. 27, 1564-1570.
Arnold W. M., Schwan H. P. and ZinnnerruauuU.. 1987. Snr face couductance and other properties of latex particles measn red by electro rotatiou. 1. Phys. Chem 91, 5093-5098.
Huang Y. and Pethig R. 1991. Electrode desigu for negative die1ectrophoresis. Meas. Sci. Techno!. 2, 1142-1146.
Griffit HA. aud Cooper J. 1998. Single-cel! measurements of human neutrophíl activation using electro rotatiou. Anal. Chem 70, 2607-2612.
Gascoyne P. Rc., Waug X-B., Huaug Y. and Becker F. F.. 1997. Dielectrophoretic separatiou of caucer cells from blooc LIEEE Traus. on Ind. Appl. 33 (3), 670-678.
Huaug Y., Wang X-B., Tame J. A. aud Pethig R. 1993. Electrokinetic behaviour of colloidal particles in travelling electricfields: sludies using yeast celIs. J. Phys. D: Appl. Phys. 26, 1528-1535.
Pethig R.. 1984. Dielectricproperties of biological materials: Biophysical audmedical applications. IEEE Trans. Electr. Insul EI-19 (5), 453-474.
Waug X-B., Huaug Y., H61zeIR, Burt J. P. H. aud Pethig R.. 1993. The oretical audex perimeutal investigations if the iuter dependence of the dielectric, dielectro phoretic and electrorotatioual behavionr of colloidal particles. J. Phys. D: Appl. Phys. 26, 312-322.
Fernández Morales F. H., Duarte J. E. Y Samitier J.. "Micro-belt convey or oflatex microspheresl!. 17th Symposium on Microelectronics Technology and Dev/ces, SBM/ cro 2002. Septiembre 9 a14 de 2002 .Port oAlegre, Brasil
Nishíoka M., Katsura S.,HíranoK and Mízuno A. 1997. Evaluations of cell characteristics by step-wíse orientationa1 rotationn singop to electrostaticrni cromanipulation. lEEE Trans. on lnd. App 133, 1381-1388.
Po WH. A.. 1951. The motion and precipitation of suspend oids in divergent electric fields. J. of Appl. Phys. 22, 869-871.
Quinn C. M., Archer G. P., Betts W. B. and O'Neill J. G.. 1996. Dose-dependent dielectrophoretic response of Cryptosporodiumoocyst streated whit ozone. Letters in Applied Microbiology 22, 224-228.
Lee S-W, Choi J-H. and Kim Y-K. .1995. Design of a biological cell fusion device. Transducers '95-Eurosensors IX. 377-380.
Gascoyue P. RC., Wang X-B., Huang Y. and Becker F. F.. 1997. Dielectrophoretic separation of cancer cells from blood. lEEE Trans. on lnd. Appl. 33(3), 670-678.
Petlrig R, Huang Y. Wang X-B. and Burtl P. H.. 1992. Positive and negative dielectrophoretic collection of colloidal partides using interdigitated castellatedr nicro electrodes. l Phys. D: Appl. Phys. 24, 881-888.
Fubr G. and Wagner B.. 1994. Electric field mediated cellmanipulation in híghly conductive culture media. Micro-system technologies'9 4I4th lnternational Conference on Micro, Electro, Opto, Mechanical Systems and Components. Berlin, Germany. 407-416.
Washizu M.. 1990. Electrostatic manipu1atiou of biological objects. Jouma1 of Electrostatics 25, 109-123.
Wang X-B., Huang Y., Gascoyne P. R. C. and Becker F. F.. 1997. Dielectrophoretic manipulation of particles. IEEE Trans. onlnd. App!. 33(3), 660-669.
Barrie G., Paul R, Kaler K. and Jones T. B.. 1995. Low-frequency micromotion of DEP-Levitated bioparticles, II: Spectral and stability analysis. Joumal of Colloid and Interface Science 175, 97-107.
Fuln G., Müller T., Schnelle T., Hagedorn R, Voigt A. and FiedIerS.. 1994. Radio-frequeucy microtools for partic1e and livingcel! manipnlation. Natur wissens chaften 81, 528-535.
Cornmings E.. 2003. Streaming dielectrophoresis for contiunous: fIow microfiuidic devices. En: IEEE in Medicine and Biology Magazine 22, 75-84.
Park J. and Lakes R.. 1992. Biomaterials: An introductiou. 2nd Editiou. Ed. Pleunm Press. New York
Panl R, Kaler K. V. L. S. and Jones T. B.. 1993. An onequilibrinm statistical mechanical calculation of the surface conductance of the electrical donble layer of biological cel!sandits application to dielectrophoresis. The Jonmal of Physical Chemistry 97 (18), 4745-4755.
Pohl H. A.. 1958. Some effects of nonn niforru fields on dielectrics. J. App!. Phys. 29, 1182-1188.
Pohl H. A. and Pethig R ..1977. Dielectric measurements using non-uniform electric field (dielectro phoretic) effects. 1. Phys. E: Sci. Instruru. lO, 190-193. Corrigendnm 883.
Mü1ler T., Gerardiuo A., Schnelle T., Shírley S. G., Fuhr G., De Gasperis G., Leo Ili Rand Bordo Ili F.. 1995. High-frequency electric-field trap for micron and submi cronparticles. llN novo Cimeuto 17 D. 425-432.
Talary M. S., Burt J. P. H., Tame J. A. and Pethig R.. 1996. Electro manipulation and separatiou of cell susing travelling electric fields. J. Phys. D: Appl. Phys. 29, 2198-2203.
Stephens M, Talary M., Pethig R., Bumett A. Y Milis K.. 1996. The dielectro phoresi seurichment of CD 34+cells from peripheral blood stem cell harvests. Bone Marrow Transplantation 18, 777-782
Pohl H. A. 1978. Dielectrophoresis: The behavior of neutral matterin non unifor ruelectric fields. Ed. Cambridge University Press. London.
Fuhr G. and Shirley S. G.. 1998. Biological application of microstructures. Topics in curreut chemistry 194, 83-116.
Fuhr G., Schnelle T., Müller T., Glasse,. H., Lisec T. aud Waguer B.. 1995. Positioning and manipulation of cells and microparticles using miniatu.lized electrie field trapsaud travelliug waves. Seusors and materials 7, 131-146.
Hughes M. P., Waug X-B., Becker F. F., Gascoyne P-R. C. aud Pethig R 1994. Computer-aided analyses of electric fields used in electro rotatiou studies. J. Phys. D: Appl. Phys. 27, 1564-1570.
Arnold W. M., Schwan H. P. and ZinnnerruauuU.. 1987. Snr face couductance and other properties of latex particles measn red by electro rotatiou. 1. Phys. Chem 91, 5093-5098.
Huang Y. and Pethig R. 1991. Electrode desigu for negative die1ectrophoresis. Meas. Sci. Techno!. 2, 1142-1146.
Griffit HA. aud Cooper J. 1998. Single-cel! measurements of human neutrophíl activation using electro rotatiou. Anal. Chem 70, 2607-2612.
Gascoyne P. Rc., Waug X-B., Huaug Y. and Becker F. F.. 1997. Dielectrophoretic separatiou of caucer cells from blooc LIEEE Traus. on Ind. Appl. 33 (3), 670-678.
Huaug Y., Wang X-B., Tame J. A. aud Pethig R. 1993. Electrokinetic behaviour of colloidal particles in travelling electricfields: sludies using yeast celIs. J. Phys. D: Appl. Phys. 26, 1528-1535.
Pethig R.. 1984. Dielectricproperties of biological materials: Biophysical audmedical applications. IEEE Trans. Electr. Insul EI-19 (5), 453-474.
Waug X-B., Huaug Y., H61zeIR, Burt J. P. H. aud Pethig R.. 1993. The oretical audex perimeutal investigations if the iuter dependence of the dielectric, dielectro phoretic and electrorotatioual behavionr of colloidal particles. J. Phys. D: Appl. Phys. 26, 312-322.
Fernández Morales F. H., Duarte J. E. Y Samitier J.. "Micro-belt convey or oflatex microspheresl!. 17th Symposium on Microelectronics Technology and Dev/ces, SBM/ cro 2002. Septiembre 9 a14 de 2002 .Port oAlegre, Brasil