Vol. 22 No. 1 (2023): Revista UIS Ingenierías
Articles

Influence of Bacteria on Self-Healing Concrete

Socrates Pedro Muñoz-Pérez
Universidad Señor de Sipan
Jorge Carlos-Sánchez
Universidad Señor de Sipan
Miguel Peralta-Sánchez
Universidad Señor de Sipan

Published 2023-01-20

Keywords

  • Bacteria,
  • bacillus;,
  • mechanical properties,
  • microbial elements,
  • subtillis,
  • ureolysis,
  • self-healing,
  • cells,
  • crack healing,
  • cracking,
  • calcium carbonate
  • ...More
    Less

How to Cite

Muñoz-Pérez, S. P., Carlos-Sánchez , J. ., & Peralta-Sánchez , M. (2023). Influence of Bacteria on Self-Healing Concrete. Revista UIS Ingenierías, 22(1), 69–86. https://doi.org/10.18273/revuin.v22n1-2023007

Abstract

This document contemplates a critical reflection of the technological advances in biological and chemical tests that concrete is subjected to through the incorporation of bacteria, with the aim of knowing the different microbial elements that have biomineralization properties capable of self-healing in concrete. Likewise, the methods and applications of bacteria in concrete were reviewed in order to improve its mechanical properties to the different demands placed on modern structures, and at the same time contribute to the reduction of gases that are harmful to the environment. In the development of this manuscript, 80 articles indexed between the years 2017 to 2021 were reviewed, distributed as follows, 51 in Scopus, 17 in Ebsco, and 12 in SciencieDirect, pointing out and describing that microbial concrete has a promising approach in the future. Near. The results achieved with the incorporation of the different Bacillus bacteria, such as Bacillus subtillis, B, cohnii, B. pasteurii, B. pseudofirmus, B. megaterium among others, in different concentrations of cells / ml., Showed great effectiveness in the crack healing, also increasing resistance to compression, bending and traction in concrete. Based on the literary review, it is concluded that the microbial precipitation of calcium carbonate by ureolysis in the concrete matrix mitigates cracking, improves strength, increases durability and, therefore, reduces costs in repairing structures.

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References

  1. T. Zheng, C. Qian, “Self-Healing of Later-Age Cracks in Cement-Based Materials by Encapsulation-Based Bacteria,” Journal of Materials in Civil Engineering, vol. 32, nº 11, p. 04020341, 2020, doi: https://doi.org/10.1061/(ASCE)MT.1943-5533.0003437
  2. M. Fahimizadeh, A. Diane Abeyratne, L. S. Mae, R. K. R. Singh, P. Pasbakhsh, “Biological Self-Healing of Cement Paste and Mortar by Non-Ureolytic Bacteria Encapsulated in Alginate Hydrogel Capsules,” Materials, vol. 13, no. 17, p. 3711, 2020, doi: https://doi.org/10.3390%2Fma13173711
  3. M. Rauf, W. Khaliq, R. A. Khushnood, I. Ahmed, “Comparative performance of different bacteria immobilized in natural fibers for self-healing in concrete,” Construction and Building Materials, vol. 258, p. 119578, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.119578
  4. M. Zamani, S. Nikafshar, A. Mousa, A. Behnia, “Bacteria encapsulation using synthesized polyurea for self-healing of cement paste,” Construction and Building Materials, vol. 249, p. 118556, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.119578
  5. E. Ahn, H. Kim, S.-H. Sim, S. W. hin y M. Shin, “Principles and Applications of Ultrasonic-Based Nondestructive Methods for Self-Healing in Cementitious Materials,” Materials, vol. 10, nº 3, p. 278, 2017, doi: https://doi.org/10.3390/ma10030278
  6. T. Zheng, C. Qian, “Self-Healing of Later-Age Cracks in Cement-Based Materials by Encapsulation-Based Bacteria,” Journal of Materials in Civil Engineering, vol. 32, nº 1-10, p. 04020341, 2020, doi: https://doi.org/10.1061/(ASCE)MT.1943-5533.0003437
  7. M. Rajczakowska, K. Habermehl-Cwirzen, H. Hedlund, A. Cwirzen, “Autogenous Self-Healing: A Better Solution for Concrete,” Journal of Materials in Civil Engineering, vol. 31, nº 9, p. 03119001, 2019, doi: https://doi.org/10.1061/(ASCE)MT.1943-5533.0002764
  8. M. Seifan, A. Ebrahiminezhad, Y. Ghasemi, A. K. Samani, A. Berenjian, “Amine-modified magnetic iron oxide nanoparticle as a promising carrier for application in bio self-healing concrete” Biotechnological products and process engineering, vol. 102, nº 1, pp. 175-184, 2017, doi: https://doi.org/10.1007/s00253-017-8611-z
  9. L. Tan, B. Reeksting, V. Ferrandiz-Mas, A. Heath, S. Gebhard, K. Paine, “Effect of carbonation on bacteria-based self-healing of cementitious composites,” Construction and Building Materials, vol. 257, p. 119501, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.119501
  10. K. K. Maurya, T. Sonker, A. Rawat, “Sustainable concrete construction by microorganism and monitoring using EMI technique: A review,” Materials Today: Proceedings, vol. 32, pp. 670-676, 2020, doi: https://doi.org/10.1016/j.matpr.2020.03.169
  11. B. Chattopadhyay, “Genetically-enriched microbe-facilitated self-healing nano-concrete,” Smart Nanoconcretes and Cement-Based Materials, pp. 461-483, 2020, doi: https://doi.org/10.1016/B978-0-12-817854-6.00020-9
  12. S. Han, I. ang, E. . K. Choi, W. Park, C. Yi. N. Hung, “Bacterial Self-Healing Performance of Coated Expanded Clay in Concrete,” Journal of Environmental Engineering, vol. 146, no. 7, p. 04020072, 2020, doi: https://doi.org/10.1061/(ASCE)EE.1943-7870.0001713
  13. T. V. Mullem, E. Gruyaert, R. Caspeele and N. De Belie, “First Large Scale Application with Self-Healing Concrete in Belgium: Analysis of the Laboratory Control Tests,” Materials, vol. 13, pp. 1-20, 2020, doi: https://doi.org/10.3390/ma13040997
  14. S. Guzlena, G. Sakale, “Self-healing concrete with crystalline admixture – a review,” IOP Conf. Ser.: Mater. Sci. Eng., pp. 660, 2019, doi: https://doi.org/10.1088/1757-899X/660/1/012057
  15. C. Sonali Sri Durga, N. Ruben, M. Sri Rama Chand, C. Venkatesh, “Performance studies on rate of self-healing in bio concrete,” Materials Today: Proceedings, vol. 27, pp. 158-162, 2019, doi: https://doi.org/10.1016/j.matpr.2019.09.151
  16. Á. González, A. Parraguez, L. Corvalán, N. Correa, J. Castro, C. Stuckrath, M. González, “Evaluation of Portland and Pozzolanic cement on the self-healing of mortars with calcium lactate and bacteria,” Construction and Building Materials, vol. 257, p. 119558, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.119558
  17. A. Soysal, J. Milla, G. M. King, M. Hassan, T. Rupnow, “Evaluating the Self-Healing Efficiency of Hydrogel-Encapsulated Bacteria in Concrete,” Journal of the Transportation Research Board, p. 0361198120917973, 2020, doi: https://doi.org/10.1177/036119812091797
  18. S. Bansal, R. K. Tamang, P. Bansal, P. Bhurtel, “Biological Methods to Achieve Self-healing in Concrete,” Advances in Structural Engineering and Rehabilitation, vol. 38, pp. 63-71, 2019, doi: https://doi.org/10.1007/978-981-13-7615-3_5
  19. S. Morsali, G. Yucel Isildar, Z. Hamed Zar Gari, A. Tahni, “The application of bacteria as a main factor in self healing concrete technology,” Journal of Building Pathology and Rehabilitation, vol. 4, no. 1, 2019, doi: https://doi.org/10.1007/s41024-019-0045-9
  20. C. Litina, G. Bumanis, G. Anglani, M. Dude, R. Maddalena, M. Amenta, S. Papaioannou, G. Pérez, J. L. García, Calvo, E. Asensio, R. Beltrán Cobos, F. Tavares, Pinto, A. Augonis, R. Davies, A. Guerrero, M. Sánchez, Moreno, T. Stryszewska , I. Karatasios, J.-M. Tulliani, P. Antonac, D. Bajare, A. Al-Tabbaa, “Evaluation of Methodologies for Assessing Self-Healing Performance of Concrete with Mineral Expansive Agents: An Interlaboratory Study,” Materials, vol. 14, nº 8, p. 2024, 2021, doi: https://doi.org/10.3390/ma14082024
  21. S. P. Saridhe, T. Selvaraj, “Microbial precipitation of calcium carbonate in cementitious materials – A critical review,” Materials Today: Proceedings, vol. 43, pp. 1232-1240, 2021, doi: https://doi.org/10.1016/j.matpr.2020.08.762
  22. R. Jakubovskis, A. Jankute , J. Urbonavicius y V. Gribniak, “Analysis of mechanical performance and durability of self-healing biological concrete,” Construction and Building Materials, vol. 260, p. 119822, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.119822
  23. M. Gao, J. Guo, H. Cao, H. Wang, X. Xiong, R. Krastev, K. Nie, H. Xu, L. Liu, “Immobilized bacteria with pH-response hydrogel for self-healing of concrete,” Journal of Environmental Management, vol. 261, p. 110225, 2020, doi: https://doi.org/10.1016/j.jenvman.2020.110225
  24. K. Vijay, M. Murmu, “Effect of calcium lactate and Bacillus subtilis bacteria on properties of concrete and self-healing of cracks,” Int. J. Structural Engineering, vol. 10, no. 3, pp. 217-231, 2020.
  25. Z. Prošek, P. Ryparová, P. Tesárek, “Application of Bacteria as Self-Healing Agent for the Concrete and Microscopic Analysis of the Microbial Calcium Precipitation Process,” Key Engineering Materials, vol. 846, pp. 237-242, 2020.
  26. L. Jiang, G. Jia, C. Jiang, Z. Li, “Sugar-coated expanded perlite as a bacterial carrier for crack-healing concrete applications,” Construction and Building Materials, vol. 232, p. 117222, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2019.117222
  27. P. Kumar Jogi, T. Vara Lakshm, “Self healing concrete based on different bacteria: A review,” Materials Today: Proceedings, vol. 43, pp. 1246-1252, 2021, doi: https://doi.org/10.1016/j.matpr.2020.08.765
  28. S. Mondal, A. (Dey) Ghosh, “Review on microbial induced calcite precipitation mechanisms leading to bacterial selection for microbial concrete,” Construction and Building Materials, vol. 225, pp. 67-75, 2019, doi: https://doi.org/10.1016/j.conbuildmat.2019.07.122
  29. S. Joshi, S. Goyal, A. Mukherjee y M. S. Reddy, “Microbial healing of cracks in concrete: a review,” Journal of Industrial Microbiology & Biotechnology, vol. 44, nº 11, pp. 1551-1525, 2017.
  30. S. C. Chuo, S. F. Mohamed, S. H. Mohd Setapar, A. Ahmad, M. Jawaid, W. A. Wani, A. A. Yaqoob y M. N. Mohamad Ibrahim, “Insights into the Current Trends in the Utilization of Bacteria for Microbially Induced Calcium Carbonate Precipitation,” Materials, vol. 13, nº 21, p. 4993, 2020.
  31. Y. Su , T. Zheng, C. Qian, “Application potential of Bacillus megaterium encapsulated by low alkaline sulphoaluminate cement in self-healing concrete,” Construction and Building Materials, vol. 273, p. 121740, 2020.
  32. P. Ryparová, P. Tesárek, H. Schreiberová and Z. Prošek, “The effect of temperature on bacterial self-healing processes in building materials,” Development of Materials Science in Research and Education (DMSRE29, 2020, doi: https://doi.org/10.1088/1757-899X/726/1/012012
  33. P. Singh, I. Kaur, N. Singh, “A review of different bacteria carriers used in self-healing mechanism,” Materials Today: Proceedings, vol. 32, pp. 995-960, 2020.
  34. S. Lu, M. Chen, Y. Dang, L. Cao, J. He, J. Zhong, “Bacterial self-healing cement-based materials: Mechanism at nanoscale,” AIP Advances, vol. 9, nº 10, p. 105312, 2019.
  35. Y. Su, C. Qian, Y. Rui, J. Feng, “Exploring the coupled mechanism of fibers and bacteria on self-healing concrete from bacterial extracellular polymeric substances (EPS),” Cement and Concrete Composites, vol. 116, p. 103896, 2021.
  36. M. Esaker, O. Hamza, A. Souid, D. Elliott, “Self-healing of bio-cementitious mortar incubated within neutral and acidic soil,” Materials and Structures, vol. 54, no. 96, 2021, doi: https://doi.org/10.1617/s11527-021-01690-1
  37. C. S. Sri Durga, N. Ruben, M. Sri Rama Chand, M. Indira, C. Venkatesh, “Comprehensive microbiological studies on screening bacteria for self-healing concrete,” Materialia, vol. 15, p. 101051, 2021.
  38. K. M. Osman, F. M. Taher, A. Abd EL-Tawab, A. S. Faried, “Role of different microorganisms on the mechanical characteristics, self-healing efficiency, and corrosion protection of concrete under different curing conditions,” Journal of Building Engineering, vol. 41, p. 102414, 2021.
  39. J. Intarasoontron, W. Pungrasmi, P. Nuaklong, P. Jongvivatsakul, S. Likitlersuang, “Comparing performances of MICP bacterial vegetative cell and microencapsulated bacterial spore methods on concrete crack healing,” Construction and Building Materials, vol. 302, p. 124227, 2021, doi: https://doi.org/10.1016/j.conbuildmat.2021.124227
  40. C. Qian, T. Zheng, X. Zhang and Y. Su, “Application of microbial self-healing concrete: Case study,” Construction and Building Materials, vol. 290, p. 123226, 2021, doi: https://doi.org/10.1016/j.conbuildmat.2021.123226
  41. P. Ryparova, Z. Prošek , H. Schreiberová, P. Bílý, P. Tesárek, “The role of bacterially induced calcite precipitation in self-healing of cement paste,” Journal of Building Engineering, vol. 39, p. 102299, 2021.
  42. M. Rauf, W. Khaliq, R. A. hushnood, I. Ahmed, “Comparative performance of different bacteria immobilized in natural fibers for self-healing in concrete,” Construction and Building Materials, vol. 258, p. 119578, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.119578
  43. J. Zhang, C. Zhao, A. Zhou, C. Yang, L. Zhao, Z. Li, “Aragonite formation induced by open cultures of microbial consortia to heal cracks in concrete: Insights into healing mechanisms and crystal polymorphs,” Construction and Building Materials, vol. 224, pp. 815-822, 2019.
  44. M. Pourfallahi, A. Nohegoo-Shahvari, M. Salimizadeh, “Effect of direct addition of two different bacteria in concrete as self-healing agent,” Structures, vol. 28, pp. 2646-2660, 2020.
  45. J. Feng, Y. Su, C. Qian, “Coupled effect of PP fiber, PVA fiber and bacteria on self-healing efficiency of early-age cracks in concrete,” Construction and Building Materials, vol. 228, p. 116810, 2019, doi: https://doi.org/10.1016/j.conbuildmat.2019.116810
  46. H. W. Kua, S. Gupta, A. N. Aday, W. V. Srubar, “Biochar-immobilized bacteria and superabsorbent polymers enable self-healing of fiber-reinforced concrete after multiple damage cycles,” Cement and Concrete Composites, vol. 100, pp. 35-52, 2019, doi: http://dx.doi.org/10.1016/j.cemconcomp.2019.03.017
  47. S. Gupta, H. W. Kua, S. D. Pang, “Healing cement mortar by immobilization of bacteria in biochar: An integrated approach of self-healing and carbon sequestration,” Cement and Concrete Composites, vol. 86, pp. 238-254, 2018.
  48. J. Xu, X. Wang, “Self-healing of concrete cracks by use of bacteria-containing low alkali cementitious material,” Construction and Building Materials, vol. 167, pp. 1-14, 2018.
  49. M. Alazhari, T. Sharma, A. Heath, R. Cooper y K. Paine, “Application of expanded perlite encapsulated bacteria and growth media for self-healing concrete,” Construction and Building Materials, vol. 160, pp. 610-619, 2018, doi: https://doi.org/10.1016/j.conbuildmat.2017.11.086
  50. J. Xu, X. Wang, “Self-healing of concrete cracks by use of bacteria-containing low alkali cementitious material,” Construction and Building Materials, vol. 167, pp. 1-14, 2018.
  51. J. Xu, X. Wang, B. Wang, “Biochemical process of ureolysis-based microbial CaCO3 precipitation and its application in self-healing concrete,” Applied Microbiology and Biotechnology, vol. 102, nº 7, pp. 3121-3132, 2018.
  52. J. Wang, A. Mignon, G. Trenson, S. Van Vlierberghe, N. Boon N. De Belie, “A chitosan-based pH-responsive hydrogel for encapsulation of bacteria for self-sealing concret,” Cement and Concrete Composites, vol. 93, pp. 309-322, 2018, doi: https://doi.org/10.1016/j.cemconcomp.2018.08.007
  53. H. Amer Algaifi, S. Abu Bakar, A. Rahman Mohd. Sam, M. Ismail, A. Razin Zainal Abidin, S. Shahir, W. Ali Hamood Altowayti, “Insight into the role of microbial calcium carbonate and the factors involved in self-healing concrete,” Construction and Building Materials, vol. 254, p. 119258, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.119258
  54. V. A. Rajani, S. K. Sunil, S. Jitendra, M. Dhananjay, “Compressive strength of bacterial concrete by varying concentrations of E.Coli and JC3 bacteria for Self-Healing Concrete,” International Journal of Innovative Technology and Exploring Engineering, vol. 9, nº 1, pp. 3659-3661, 2019.
  55. H. Rong, G. Wei, G. Ma, Y. Zhang, X. Zheng, L. Zhang and R. Xu, “Influence of bacterial concentration on crack self-healing of cement-based materials,” Construction and Building Materials, vol. 244, p. 118372, 2020.
  56. N. Hosseini Balam, D. Mostofinejad, M. Eftekhar, “Effects of bacterial remediation on compressive strength, water absorption, and chloride permeability of lightweight aggregate concrete,” Construction and Building Materials, vol. 145, pp. 107-116, 2017.
  57. R. Siddique, A. Jameel, M. Singh, D. Barnat-Hunek, Kunal, A. Aït-Mokhtar, R. Belarbi, A. Rajor, “Effect of bacteria on strength, permeation characteristics and micro-structure of silica fume concrete,” Construction and Building Materials, vol. 142, pp. 92-100, 2017.
  58. O. Hamza, M. Esaker, D. Elliott, A. Souid, “The effect of soil incubation on bio self-healing of cementitious mortar,” Materials Today Communications, vol. 24, p. 100988, 2020.
  59. S. Farhadi, S. Ziadloo, “Self-Healing Microbial Concrete - A Review,” Materials Science Forum, vol. 990, pp. 8-12, 2020, doi: https://doi.org/10.4028/www.scientific.net/MSF.990.8
  60. R. Vashisht, S. Attri, D. Sharma, A. Shukla, G. Goel, “Monitoring biocalcification potential of Lysinibacillus sp. isolated from alluvial soils for improved compressive strength of concrete,” Microbiological Research, vol. 207, pp. 226-231, 2018, doi: https://doi.org/10.1016/j.micres.2017.12.010
  61. S. Basha, L. K. Lingamgunta, J. Kannali, S. K. Gajula, R. Bandikari, S. Dasari, V. Dalavai, P. Chinthala, P. Gundala, P. Kutagolla, V. K. Balaji, “Subsurface endospore-forming bacteria possess bio-sealant properties,” Scientific Reports, vol. 8, nº 1, 2018.
  62. P. Reddy, B. Kavyateja, “Experimental Study on Strength Parameters of Self Repairing Concrete,” Annales de Chimie: Science des Materiaux, vol. 43, nº 5, pp. 305-310, 2019.
  63. K. Vijay, M. Murmu, S. V. Deo, “Bacteria based self healing concrete – A review,” Construction and Building Materials, vol. 152, pp. 1008-1014, 2017, doi: https://doi.org/10.1016/j.conbuildmat.2017.07.040
  64. S. S. Lucas, C. Moxham, E. Tziviloglou, H. Jonkers, “Study of self-healing properties in concrete with bacteria encapsulated in expanded clay,” Science and Technology of Materials, vol. 30, pp. 93-98, 2018, doi: https://doi.org/10.1016/j.stmat.2018.11.006
  65. L. Dembovska, D. Bajare, A. Korjakins, D. Toma, E. Jakubovica, “Preliminary research for long lasting self-healing effect of bacteria-based concrete with lightweight aggregates,” 2019, doi: https://doi.org/10.1088/1757-899X/660/1/012034
  66. W. Pungrasmi, J. Intarasoontron, P. Jongvivatsakul, S. Likitlersuang, “Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete,” Scientific Reports, vol. 9, nº 1, 2019.
  67. J. Feng, Y. Su, C. Qian, “Coupled effect of PP fiber, PVA fiber and bacteria on self-healing efficiency of early-age cracks in concrete,” Construction and Building Materials, vol. 228, p. 116810, 2019, doi: https://doi.org/10.1016/j.conbuildmat.2019.116810
  68. S. Jena, B. Basa, C. Panda, N. K. Sahoo, “Impact of Bacillus subtilis bacterium on the properties of concrete,” Materials Today: Proceedings, vol. 32, pp. 651-656, 2020.
  69. A. Chithambar Ganesh, M. Muthukannan, R. Malathy, C. Ramesh Babu, “An Experimental Study on Effects of Bacterial Strain Combination in Fibre Concrete and Self-Healing Efficiency,” KSCE Journal of Civil Engineering, vol. 23, nº 10, pp. 4368-4377, 2019, doi: https://doi.org/10.1007/s12205-019-1661-2
  70. K. Vijay, M. Murmu, “Self repairing of concrete cracks by using bacteria and basalt fiber,” SN Applied Sciences, vol. 1, nº 11, 2019.
  71. C. Manvith Kumar Reddy, B. M. D. Ramesh, K. Reddy, “Influence of bacteria Bacillus subtilis and its effects on flexural strength of concrete,” Materials Today: Proceedings, vol. 33, pp. 4206-4211, 2020.
  72. J. Feng, B. Chen, W. Sun, Y. Wang, “Microbial induced calcium carbonate precipitation study using Bacillus subtilis with application to self-healing concrete preparation and characterization,” Construction and Building Materials, vol. 280, p. 122460, 2021, doi: https://doi.org/10.1016/j.conbuildmat.2021.122460
  73. P. Pachaivannan, C. Hariharasudhan, M. Mohanasundram, M. Anitha Bhavani, “Experimental anaylsis of self healing properties of bacterial concrete,” Materials Today: Proceedings, vol. 33, pp. 3148-3154, 2020.
  74. I. Rohini, R. Padmapriya, “Effect of bacteria subtilis on e-waste concrete,” Materials Today: Proceedings, vol. 42, pp. 465-474, 2021, doi: https://doi.org/10.1016/j.matpr.2020.10.192
  75. N. Nain, R. Surabhi, N. Yathish, V. Krishnamurthy, T. Deepa, S. Tharannum, “Enhancement in strength parameters of concrete by application of Bacillus bacteria,” Construction and Building Materials, vol. 202, pp. 904-908, 2019.
  76. N. Shaheen, R. A. Khushnood, W. Khaliq, H. Murtaza, R. Iqbal, M. H. Khan, “Synthesis and characterization of bio-immobilized nano/micro inert and reactive additives for feasibility investigation in self-healing concrete,” Construction and Building Materials, vol. 226, pp. 492-506, 2019.
  77. M. S. Jafarnia, M. K. Saryazdi, S. M. Moshtaghioun, “Use of bacteria for repairing cracks and improving properties of concrete containing limestone powder and natural zeolite,” Construction and Building Materials, vol. 242, p. 118059, 2020, doi: https://doi.org/10.1016/j.conbuildmat.2020.118059
  78. H. Xu, J. Lian,, M. Gao, D. Fu, Y. Yan, “Self-Healing Concrete Using Rubber Particles to Immobilize Bacterial Spores,” Materials, vol. 12, nº 14, p. 2313, 2019, doi: https://doi.org/10.3390/ma12142313
  79. T. Shanmuga Priya, N. Ramesh, A. Agarwal, S. Bhusnur, K. Chaudhary, “Strength and durability characteristics of concrete made by micronized biomass silica and Bacteria-Bacillus sphaericus,” Construction and Building Materials, vol. 226, pp. 827-838, 2019, doi: https://doi.org/10.1016/j.conbuildmat.2019.07.172
  80. H. Kalhori, R. Bagherpour, “Application of carbonate precipitating bacteria for improving properties and repairing cracks of shotcrete,” Construction and Building Materials, vol. 148, pp. 249-260, 2017, doi: https://doi.org/10.1016/j.conbuildmat.2017.05.074