Keywords
Gram-Negative Bacteria
Multidrug Resistance
Anti-Infective Agents
Beta-Lactamases
Multidrug Efflux Pump Genes
How to Cite
Abstract
Introduction: the rise of bacteria resistant to multiple drugs underscores the need to enhance antimicrobial therapies. Efflux pumps, which decrease the concentration of antimicrobials in bacteria, play a key role in resistance. Objective: The objective of this study is to evaluate the effect of the efflux pump inhibitor Phenylalanine-arginine β-naphthylamide on bacterial resistance to antibiotics ceftazidime, chlortetracycline, ciprofloxacin, ceftriaxone, ampicillin, and gentamicin in gram-negative bacilli with multiple ESBL genes isolated. Materials and Methods: an experimental study was conducted to analyze multi-resistant gram-negative bacillus isolates associated with infections from a healthcare institution in Boyacá. From 81 bacterial isolates, antibiotic resistance genes were detected in 70 strains through conventional polymerase chain reaction and Sanger sequencing. Results: the analysis of 70 isolates revealed the presence of genetic determinants for beta-lactam antibiotic resistance such as blaTEM1, blaSHV1, blaSHV2 and blaCTM-X, with a predominance of the blaSHV gene. Conclusions: the interaction assay showed that in multi-resistant strains, combination therapy with efflux pump inhibitors could be an alternative for treatment. However, due to the diverse nature of efflux pumps, other families of inhibitors should be evaluated to identify those effective across a larger number of strains.
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