Keywords
Enzyme-linked immunosorbent assay
diagnosis
fascioliasis
fasciola hepatica
standardization
humans
sheep
cattle
diagnosis
fascioliasis
fasciola hepatica
standardization
humans
sheep
cattle
How to Cite
Sierra Barcárcel, R., Martínez Vega, R. A., Gutiérrez Marín, R., Colmenares, C. D., & Uribe, N. (2017). Standardization of ELISA for the diagnosis of bovine, ovine and human fasciolosis. Salud UIS, 49(4), 549–555. https://doi.org/10.18273/revsal.v49n4-2017004
Abstract
Introduction: Fascioliasis represents a serious problem for livestock production and human health in the world. In Colombia, it mainly affects bovine and sheep cattle, with few cases reported in humans. Objective: To standardize an Enzyme-Linked ImmunoSorbent test (ELISA) in bovine, ovine, and human individuals as a screening tool of fascioliasis. Methods: 50 bovine, 50 ovine and 39 human samples with fascioliasis confirmed by serology and stool test were used. Sensitivity, specificity and concordance were calculated. Results: Sensitivity of the ELISA in humans, sheep and cattle was 100 % and specificity was of 97%, 85.2% and 96.2%, respectively; Cohen´s Kappa coefficient was >0.8 in all groups. Conclusions: Standardized ELISA test for bovine, ovine and human fascioliasis exhibited an excellent sensitivity and good specificity. We propose to carry out a large-scale validation for its ulterior use as a screening tool for the diagnosis of this parasitic infection.
References
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Vitae. 2013; 56: 1-10.
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National Congress of Veterinary Parasitology & National Symposium on “Towards Food Security through Sustainable Animal Production and Integrated Parasite Management”; 2014 Feb 5-7; Thrissur, India. College of
Veterinary & Animal Sciences; 2014. pp. 164-174.
12. Shafiei R, Sarkari B, Sadjjadi SM. Performance of a 27 kDa Fasciola hepatica Antigen in the Diagnosis of
Human Fascioliasis. J Lab Physicians. 2015; 7(1): 17-20. DOI: 10.4103/0974-2727.154781.
13. Carnevale S, Rodríguez MI, Guarnera EA, Carmona C, Tanos T, Angel SO. Immunodiagnosis of fasciolosis
using recombinant procathepsin L cystein proteinase. Diagn Microbiol Infect Dis. 2001; 41(1-2): 43-49. DOI: 10.1016/S0732-8893(01)00288-7.
14. El Ridi R, Salah M, Wagih A, William H, Tallima H, El Shafie MH, et al. Fasciola gigantica excretorysecretory products for immunodiagnosis and prevention of sheep fasciolosis. Vet Parasitol. 2007; 149(3-4): DOI: 219-228. 10.1016/j.vetpar.2007.08.024.
15. Escalante H, Davelois K, Ortiz P, Rodríguez H, Díaz E, Jara C. Western blot technique standardization of the diagnosis of human fasciolosis using Fasciola hepatica excreted-secreted antigens. Rev Peru Med Exp Salud Publica. 2011; 28(3): 454-461. DOI: 10.1590/S1726-46342011000300008.
16. Rivera-Marrero CA, Santiago N, Hillyer GV. Evaluation of immunodiagnostic antigens in the excretorysecretory
products of Fasciola hepatica. J Parasitol. 1988; 74(4): 646-652. DOI: 10.2307/3282184.
17. Espino AM, Borges A, Duménigo BE. Coproantígenos de Fasciola hepatica de posible utilidad en el diagnóstico de la fasciolosis. Rev Panam Salud Pública. 2000; 7(4): 225-231. DOI: 10.1590/S1020-49892000000400003.
18. Kim K, Yang HJ, Chung YB. Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis. Korean J Parasitol. 2003; 41(2): 121-123. DOI: 10.3347/kjp.2003.41.2.121.
19. Silva M, Gorman T, Alcaíno H. Inmunodiagnóstico de fasciolosis humana y ovina empleando una fracción de 24-29 kDa de F. hepatica obtenida mediante inmunoadsorción. Parasitol Latinoam. 2005; 60(1-2): 38-42. DOI: 10.4067/S0717-77122005000100005.
20. Lehner RP, Sewell MMH. A study of the antigen produced by adult Fasciola hepatica maintained in vitro. Paras Immunol. 1980; 2(2): 99-109. DOI: 10.1111/j.1365-3024.1980.tb00046.x.
21. Cornejo H, Oblitas F, Cruzado S, Quispe W. Evaluación de una prueba de ELISA con antígeno metabólico de Fasciola hepatica para el diagnóstico de fasciolosis humana en Cajamarca, Perú. Rev Peru Med Exp Salud Pública. 2010; 27(4): 569-574. DOI: 10.1590/S1726-46342010000400012.
22. Colmenares C, Méndez L, Díaz-Bello Z, Alarcón B. Antígeno excreción-secreción de Fasciola hepatica: ultrafiltración y aplicación en inmunodiagnóstico. Acta Bioquím Clín Latinoam. 2007; 41(2): 259-266.
23. Voller A, Bartlett A, Bidwell DE. Enzyme immunoassays for parasitic diseases. Tras Roy Soc Trop Med Hyg. 1976; 70(2): 98-106. doi:10.1016/0035-9203(76)90163-2.
24. Salimi-Bejestani MR, McGarry JW, Felstead S, Ortiz P, Akca A, Williams DJ. Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test. Res Vet Sci. 2005; 78(2): 177-181. DOI: 10.1016/j.rvsc.2004.08.005.
25. Orozco LC, Ovalle CE, Duque S, López MC, Nicholls RS. Exactitud del ELISA para el diagnóstico de fascioliasis bovina: análisis del área bajo la curva del receptor operador (ROC). Biomédica. 1996; 16(2): 122-130. DOI: 10.7705/biomedica.v16i2.902.
26. Pfister K, Daveau CH, Ambroise-Thomas P. Partial purification of somatic and excretory-secretory products of adult Fasciola hepatica and their application for the serodiagnosis of experimental and natural fascioliasis using on ELISA. Res Vet Sci. 1984; 37(1): 39-43.
27. Espino AM, Diaz A, Perez A, Finlay CM. Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak. J Clin Microb. 1998; 36(9): 2723-2726.
28. Wilches C, Jaramillo J, Muñoz D, Robledo S, Velez I. Presence of infection by Fasciola hepatica in inhabitants from the valle de San Nicolás, eastern Antioquia. Infectio. 2009; 13(2): 92-99. DOI: 10.1016/S0123-9392(09)70730-1.
2. Le TH, Van De N, Agatsuma T, Thi Nguyen TH, Nguyen QD, McManus DP, et al. Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam.
Int J Parasitol. 2008; 38(6): 725-730. DOI: 10.1016/j.ijpara.2007.10.003.
3. Mas-Coma S, Bargues MD, Valero MA, Fuentes MV. Adaptation capacities of Fasciola hepatica and their relationships with human fasciolosis: from below sea level up to the very high altitude. En: Combes C, Jourdane J. Taxonomy, ecology and evolution of metazoan parasites. Perpignan: Perpignan University Press; 2003: p.81-123.
4. Cwiklinski K, O’Neill SM, Donnelly S, Dalton JP. A prospective view of animal and human Fasciolosis. Parasite Immunol. 2016; 38(9): 558-568. DOI: 10.1111/pim.12343.
5. Benavides E. Diseño de planes racionales de control de parásitos internos de los rumiantes con base en los resultados de investigaciones sobre su dinámica poblacional. Rev Corpoica. 1996; 2: 79-88.
6. Morales A, Espino AM. Evaluation and characterization of Fasciola hepatica tegument protein extract for serodiagnosis of human fascioliasis. Clin Vaccine Immunol. 2012; 19(11): 1870-1878. DOI: 10.1128/CVI.00487-12.
7. Mas-Coma, S. Human fascioliasis. En: Cotruvo JA, Dufour A, Rees G, Bartram J, Carr R, Cliver DO, editors. Waterborne Zoonoses: Identification, causes and control. London: IWA Publishing; 2004:p.305-322.
8. Uribe N, García C. Fasciolosis, zoonosis emergente y reemergente vista desde una dimensión ambiental.
Vitae. 2013; 56: 1-10.
9. Mas-Coma S. Epidemiology of fascioliasis in human endemic areas. J Helminthol. 2005; 79 (3): 207-216. DOI: 10.1079/JOH2005296.
10. Mas-Coma S. Human fascoliasis: Epidemiological patterns in human endemic areas of South America, Africa and Asia. South Asian J Trop Med Public Health. 2004; 35(S1): 1-11.
11. Gupta SC. Fasciolosis in man and animals: an overview retrospect to historical perspective. Acts del XXIV
National Congress of Veterinary Parasitology & National Symposium on “Towards Food Security through Sustainable Animal Production and Integrated Parasite Management”; 2014 Feb 5-7; Thrissur, India. College of
Veterinary & Animal Sciences; 2014. pp. 164-174.
12. Shafiei R, Sarkari B, Sadjjadi SM. Performance of a 27 kDa Fasciola hepatica Antigen in the Diagnosis of
Human Fascioliasis. J Lab Physicians. 2015; 7(1): 17-20. DOI: 10.4103/0974-2727.154781.
13. Carnevale S, Rodríguez MI, Guarnera EA, Carmona C, Tanos T, Angel SO. Immunodiagnosis of fasciolosis
using recombinant procathepsin L cystein proteinase. Diagn Microbiol Infect Dis. 2001; 41(1-2): 43-49. DOI: 10.1016/S0732-8893(01)00288-7.
14. El Ridi R, Salah M, Wagih A, William H, Tallima H, El Shafie MH, et al. Fasciola gigantica excretorysecretory products for immunodiagnosis and prevention of sheep fasciolosis. Vet Parasitol. 2007; 149(3-4): DOI: 219-228. 10.1016/j.vetpar.2007.08.024.
15. Escalante H, Davelois K, Ortiz P, Rodríguez H, Díaz E, Jara C. Western blot technique standardization of the diagnosis of human fasciolosis using Fasciola hepatica excreted-secreted antigens. Rev Peru Med Exp Salud Publica. 2011; 28(3): 454-461. DOI: 10.1590/S1726-46342011000300008.
16. Rivera-Marrero CA, Santiago N, Hillyer GV. Evaluation of immunodiagnostic antigens in the excretorysecretory
products of Fasciola hepatica. J Parasitol. 1988; 74(4): 646-652. DOI: 10.2307/3282184.
17. Espino AM, Borges A, Duménigo BE. Coproantígenos de Fasciola hepatica de posible utilidad en el diagnóstico de la fasciolosis. Rev Panam Salud Pública. 2000; 7(4): 225-231. DOI: 10.1590/S1020-49892000000400003.
18. Kim K, Yang HJ, Chung YB. Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis. Korean J Parasitol. 2003; 41(2): 121-123. DOI: 10.3347/kjp.2003.41.2.121.
19. Silva M, Gorman T, Alcaíno H. Inmunodiagnóstico de fasciolosis humana y ovina empleando una fracción de 24-29 kDa de F. hepatica obtenida mediante inmunoadsorción. Parasitol Latinoam. 2005; 60(1-2): 38-42. DOI: 10.4067/S0717-77122005000100005.
20. Lehner RP, Sewell MMH. A study of the antigen produced by adult Fasciola hepatica maintained in vitro. Paras Immunol. 1980; 2(2): 99-109. DOI: 10.1111/j.1365-3024.1980.tb00046.x.
21. Cornejo H, Oblitas F, Cruzado S, Quispe W. Evaluación de una prueba de ELISA con antígeno metabólico de Fasciola hepatica para el diagnóstico de fasciolosis humana en Cajamarca, Perú. Rev Peru Med Exp Salud Pública. 2010; 27(4): 569-574. DOI: 10.1590/S1726-46342010000400012.
22. Colmenares C, Méndez L, Díaz-Bello Z, Alarcón B. Antígeno excreción-secreción de Fasciola hepatica: ultrafiltración y aplicación en inmunodiagnóstico. Acta Bioquím Clín Latinoam. 2007; 41(2): 259-266.
23. Voller A, Bartlett A, Bidwell DE. Enzyme immunoassays for parasitic diseases. Tras Roy Soc Trop Med Hyg. 1976; 70(2): 98-106. doi:10.1016/0035-9203(76)90163-2.
24. Salimi-Bejestani MR, McGarry JW, Felstead S, Ortiz P, Akca A, Williams DJ. Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test. Res Vet Sci. 2005; 78(2): 177-181. DOI: 10.1016/j.rvsc.2004.08.005.
25. Orozco LC, Ovalle CE, Duque S, López MC, Nicholls RS. Exactitud del ELISA para el diagnóstico de fascioliasis bovina: análisis del área bajo la curva del receptor operador (ROC). Biomédica. 1996; 16(2): 122-130. DOI: 10.7705/biomedica.v16i2.902.
26. Pfister K, Daveau CH, Ambroise-Thomas P. Partial purification of somatic and excretory-secretory products of adult Fasciola hepatica and their application for the serodiagnosis of experimental and natural fascioliasis using on ELISA. Res Vet Sci. 1984; 37(1): 39-43.
27. Espino AM, Diaz A, Perez A, Finlay CM. Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak. J Clin Microb. 1998; 36(9): 2723-2726.
28. Wilches C, Jaramillo J, Muñoz D, Robledo S, Velez I. Presence of infection by Fasciola hepatica in inhabitants from the valle de San Nicolás, eastern Antioquia. Infectio. 2009; 13(2): 92-99. DOI: 10.1016/S0123-9392(09)70730-1.
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